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TMT-based quantitative proteomic and scanning electron microscopy reveals biological and morphological changes of Staphylococcus aureus irradiated by electron beam

文献类型： 外文期刊

作者： Zheng, Qi 1 ; Chen, Zhijun 1 ; Yan, Weiqiang 1 ; Wang, Haihong 1 ; Tian, Wenhui 1 ; Feng, Dongsheng 4 ; Yue, Ling 1 ; Qi, Wenyuan 1 ; He, Xiaohua 1 ; Kong, Qiulian 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

2.ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA

3.Shanghai Acad Agr Sci, Shanghai Shuneng Irradiat Technol Co Ltd, 255 Maoyuan Rd, Shanghai 201403, Peoples R China

4.Shanghai Ctr Agriprod Qual & Safety, 779 Xianxia West Rd, Shanghai 200335, Peoples R China

关键词： Electron beam irradiation; Scanning electron microscopy; Staphylococcus aureus; TMT-Based quantitative proteomic

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2023 年 184 卷

页码：

收录情况： SCI

摘要： Electron beam irradiation is an efficient cold sterilization technique that has been widely utilized in food preservation. To understand the biological and morphological changes of microorganisms by electron beam irradiation, the D10 value, growth curve, and morphological characterization by scanning electron microscope (SEM) of Staphylococcus aureus were studied. Furthermore, Tandem mass tag (TMT)-based quantitative proteo-mic was performed to investigate the responses of Staphylococcus aureus to electron beam. The results showed that electron beam prolonged the lag phase of bacterial growth, and irradiated bacteria were more sensitive to irradiation than unirradiated bacteria. Compared to unirradiated cells, there were 53, 96, 1055, and 431 differentially expressed proteins (DEPs) detected in 1-kGy and 2-kGy irradiated cells after 0 h and 24 h incu-bation, respectively. Most of the DEPs involved in DNA repair were found to be up-regulated, whereas those involved in biofilm formation and staphylococcal enterotoxin production were down-regulated. No noticeable morphological changes were observed in irradiated S. aureus. These findings provided valuable information in the responses of S. aureus to electron beam irradiation, leading to a better understanding of the effects of electron beam irradiation on food pathogens.

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