您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Two electrochemiluminescence immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified crops

文献类型： 外文期刊

作者： Zeng, Haijuan 1 ; Zhang, Minghao 1 ; Liu, Hua 1 ; Liu, Juan 5 ; Zhu, Lemei 5 ; Feng, Dongsheng 6 ; Wang, Jinbin 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr S, Shanghai 201106, Peoples R China

3.Shanghai Coelite Agr Sci Tech Grp Co Ltd, Shanghai 201106, Peoples R China

4.Yangtze Univ, Sch Life Sci, Jingzhou 434022, Peoples R China

5.Changsha Med Univ, Sch Publ Hlth, Academician Workstat, Changsha 410219, Peoples R China

6.Shanghai Ctr Agriprod Qual & Safety, Shanghai 200335, Peoples R China

关键词： Signal-reduced or -enhanced model; Electrochemiluminescence immunosensor; CdSe; ZnS QDs; Nanocomposites; CP4-EPSPS protein

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 428 卷

页码：

收录情况： SCI

摘要： Two different models of electrochemiluminescence (ECL) immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified (GM) crops were proposed in this study. One was a signal-reduced ECL immunosensor based on nitrogen-doped graphene, graphitic carbon nitride and polyamideamine (GN-PAMAM-g-C3N4) composites as the electrochemically active substance. The other model was a signalenhanced ECL immunosensor based on a GN-PAMAM modified electrode for the detection of CdSe/ZnS quantum dots (QDs)-labeled antigens. The ECL signal responses of the reduced and enhanced immunosensors linearly decreased as the increase of the soybean RRS and RRS-QDs content in the range of 0.05% to 1.5% and 0.025% to 1.0%, with the limits of detection of 0.03% and 0.01% (S/N = 3), respectively. Both of the ECL immunosensors showed good specificity, stability, accuracy, and reproducibility in the analysis of real samples. The results indicate that the two immunosensors provide an ultra-sensitive and quantitative approach for the determination of the CP4-EPSPS protein. Due to their outstanding performances, the two ECL immunosensors could be useful tools for achieving the effective regulation of GM crops.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台