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Development of an enzyme-linked immunosorbent assay based on viral antigen capture by anti-spike glycoprotein monoclonal antibody for detecting immunoglobulin A antibodies against porcine epidemic diarrhea virus in milk

文献类型： 外文期刊

作者： Li, Rui 1 ; Wen, Ying 1 ; Yang, Lei 1 ; Qian, Qi-sheng 1 ; Chen, Xin-xin 1 ; Zhang, Jia-qing 2 ; Li, Xuewu 1 ; Xing, Bao-song 2 ; Qiao, Songlin 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Henan, Peoples R China

关键词： PEDV; IgA antibody detection; ELISA; S protein; mAb

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN：

年卷期： 2023 年 19 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPorcine epidemic diarrhea (PED), caused by PED virus (PEDV), is a severe enteric disease burdening the global swine industry in recent years. Especially, the mortality of PED in neonatal piglets approaches 100%. Maternal antibodies in milk, particularly immunoglobulin A (IgA) antibodies, are of great importance for protection neonatal suckling piglets against PEDV infection as passive lactogenic immunity. Therefore, appropriate detection methods are required for detecting PEDV IgA antibodies in milk. In the current study, we prepared monoclonal antibodies (mAbs) against PEDV spike (S) glycoprotein. An enzyme-linked immunosorbent assay (ELISA) was subsequently developed based on PEDV antigen capture by a specific anti-S mAb.ResultsThe developed ELISA showed high sensitivity (the maximum dilution of milk samples up to 1:1280) and repeatability (coefficient of variation values < 10%) in detecting PEDV IgA antibody positive and negative milk samples. More importantly, the developed ELISA showed a high coincidence rate with a commercial ELISA kit for PEDV IgA antibody detection in clinical milk samples.ConclusionsThe developed ELISA in the current study is applicable for PEDV IgA antibody detection in milk samples, which is beneficial for evaluating vaccination efficacies and neonate immune status against the virus.

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