文献类型： 外文期刊
作者： Li, Mingxu 1 ; Chen, Qianqian 1 ; Wu, Chuanhui 1 ; Li, Yiyang 1 ; Wang, Sanle 1 ; Chen, Xuelian 1 ; Qiu, Bowen 1 ; Li, Yuxin 1 ; Mao, Dongmei 1 ; Lin, Hong 2 ; Yu, Daogeng 3 ; Cao, Yajun 1 ; Huang, Zhi 1 ; Cui, Chunhong 1 ; Zhong, Zengtao 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Nanjing 210095, Peoples R China
2.Nanjing Customs, Anim Plant & Food Inspect Ctr, 39 Chuangzhi Rd, Nanjing 210019, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China
4.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China
关键词： horizontal gene transfer; integrative conjugative element; Azorhizobium caulinodans; regulation; minimal ICEAc
期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）
ISSN：
年卷期： 2022 年 13 卷 10 期
页码：
收录情况： SCI
摘要： Azorhizobium caulinodans ORS571 contains an 87.6 kb integrative and conjugative element (ICEAc) that conjugatively transfers symbiosis genes to other rhizobia. Many hypothetical redundant gene fragments (rgfs) are abundant in ICEAc, but their potential function in horizontal gene transfer (HGT) is unknown. Molecular biological methods were employed to delete hypothetical rgfs, expecting to acquire a minimal ICEAc and consider non-functional rgfs as editable regions for inserting genes related to new symbiotic functions. We determined the significance of rgf4 in HGT and identified the physiological function of genes designated rihF1a (AZC_3879), rihF1b (AZC_RS26200), and rihR (AZC_3881). In-frame deletion and complementation assays revealed that rihF1a and rihF1b work as a unit (rihF1) that positively affects HGT frequency. The EMSA assay and lacZ-based reporter system showed that the XRE-family protein RihR is not a regulator of rihF1 but promotes the expression of the integrase (intC) that has been reported to be upregulated by the LysR-family protein, AhaR, through sensing host's flavonoid. Overall, a conservative module containing rihF1 and rihR was characterized, eliminating the size of ICEAc by 18.5%. We propose the feasibility of constructing a minimal ICEAc element to facilitate the exchange of new genetic components essential for symbiosis or other metabolic functions between soil bacteria.
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