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Ohr and OhrR Are Critical for Organic Peroxide Resistance and Symbiosis in Azorhizobium caulinodans ORS571

文献类型： 外文期刊

作者： Si, Yang 1 ; Guo, Dongsen 1 ; Deng, Shuoxue 1 ; Lu, Xiuming 1 ; Zhu, Juanjuan 1 ; Rao, Bei 1 ; Cao, Yajun 1 ; Jiang, Gaof 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Natl Engn Res Ctr Organ Based Fertilizers, Jiangsu Collaborat Innovat Ctr Solid Organ Waste, Postdoctoral Stn Agr Resources & Environm,Jiangsu, Nanjing 210095, Peoples R China

3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China

关键词： Azorhizobium caulinodans; Ohr; OhrR; organic peroxide; symbiosis

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Azorhizobium caulinodans is a symbiotic nitrogen-fixing bacterium that forms both root and stem nodules on Sesbania rostrata. During nodule formation, bacteria have to withstand organic peroxides that are produced by plant. Previous studies have elaborated on resistance to these oxygen radicals in several bacteria; however, to the best of our knowledge, none have investigated this process in A. caulinodans. In this study, we identified and characterised the organic hydroperoxide resistance gene ohr (AZC_2977) and its regulator ohrR (AZC_3555) in A. caulinodans ORS571. Hypersensitivity to organic hydroperoxide was observed in an ohr mutant. While using a lacZ-based reporter system, we revealed that OhrR repressed the expression of ohr. Moreover, electrophoretic mobility shift assays demonstrated that OhrR regulated ohr by direct binding to its promoter region. We showed that this binding was prevented by OhrR oxidation under aerobic conditions, which promoted OhrR dimerization and the activation of ohr. Furthermore, we showed that one of the two conserved cysteine residues in OhrR, Cys(11), was critical for the sensitivity to organic hydroperoxides. Plant assays revealed that the inactivation of Ohr decreased the number of stem nodules and nitrogenase activity. Our data demonstrated that Ohr and OhrR are required for protecting A. caulinodans from organic hydroperoxide stress and play an important role in the interaction of the bacterium with plants. The results that were obtained in our study suggested that a thiol-based switch in A. caulinodans might sense host organic peroxide signals and enhance symbiosis.

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