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Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources

文献类型： 外文期刊

作者： Barazandeh, Mohadeseh 1 ; Shahin, Khashayar 1 ; Hedayatkhah, Abolghasem 3 ; Komijani, Majid 6 ; Mansoorianfar, Mojtaba 7 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Cultivat Base, MOST, Nanjing, Peoples R China

2.Jiangsu Acad Agr Sci, Int Phage Res Ctr, Key Lab Phage Res, Nanjing, Peoples R China

3.Royal Netherlands Inst Sea Res, Dept Marine Microbiol & Biogeochem, Den Burg, Netherlands

4.Univ Utrecht, Den Burg, Netherlands

5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Dept Freshwater & Marine Ecol, Amsterdam, Netherlands

6.Arak Univ, Fac Sci, Dept Biol, Arak, Iran

7.Sharif Univ Technol, Dept Mat Sci & Engn, Tehran, Iran

8.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China

关键词： Bacteriophage; Extensively drug resistant; Phage therapy; Pseudomonas aeruginosa

期刊名称：VETERINARY RESEARCH FORUM （ 影响因子：0.95； 五年影响因子：1.295 ）

ISSN： 2008-8140

年卷期： 2021 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (- 20.00 up to 70.00 C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.

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