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Selective breeding of cold-tolerant black soldier fly (Hermetia illucens) larvae: Gut microbial shifts and transcriptional patterns

文献类型： 外文期刊

作者： Ma, Chong 1 ; Huang, Zhijun 1 ; Feng, Xingbao 1 ; Cui, Ying 1 ; Duan, Xinyu 1 ; Zhu, Jianfeng 1 ; Tettamanti, Gianluca 3 ; Hu, Wenfeng 1 ; Tian, Ling 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China

2.Bioforte Biotechnol Shenzhen Co Ltd, Shenzhen, Peoples R China

3.Univ Insubria, Dept Biotechnol & Life Sci, I-21100 Varese, Italy

4.Univ Napoli Federico II, BAT Ctr, Interuniv Ctr Studies Bioinspired Agroenvironm Tec, I-80055 Portici, Italy

5.South China Agr Univ, Coll Food Sci, Lab Appl Microbiol, Guangzhou 510642, Guangdong, Peoples R China

关键词： Hermetia illucens L; Selective breeding; Cold tolerance; Intestinal microflora; Metagenome; Transcriptome

期刊名称：WASTE MANAGEMENT （ 影响因子：8.1； 五年影响因子：8.7 ）

ISSN： 0956-053X

年卷期： 2024 年 177 卷

页码：

收录情况： SCI

摘要： The larvae of black soldier fly (BSFL) convert organic waste into insect proteins used as feedstuff for livestock and aquaculture. BSFL production performance is considerably reduced during winter season. Herein, the intraspecific diversity of ten commercial BSF colonies collected in China was evaluated. The Bioforte colony was subjected to selective breeding at 12 degrees C and 16 degrees C to develop cold-tolerant BSF with improved production performance. After breeding for nine generations, the weight of larvae, survival rate, and the dry matter conversion rate significantly increased. Subsequently, intestinal microbiota in the cold-tolerant strain showed that bacteria belonging to Morganella, Dysgonomonas, Salmonella, Pseudochrobactrum, and Klebsiella genera were highly represented in the 12 degrees C bred, while those of Acinetobacter, Pseudochrobactrum, Enterococcus, Comamonas, and Leucobacter genera were significantly represented in the 16 degrees C bred group. Metagenomic revealed that several animal probiotics of the Enterococcus and Vagococcus genera were greatly enriched in the gut of larvae bred at 16 degrees C. Moreover, bacterial metabolic pathways including carbohydrate, lipid, amino acids, and cofactors and vitamins, were significantly increased, while organismal systems and human diseases was decreased in the 16 degrees C bred group. Transcriptomic analysis revealed that the upregulated differentially expressed genes in the 16 degrees C bred groups mainly participated in Autophagy-animal, AMPK signaling pathway, mTOR signaling pathway, Wnt signaling pathway, FoxO signaling pathway, Hippo signaling pathway at day 34 under 16 degrees C conditions, suggesting their significant role in the survival of BSFL. Taken together, these results shed lights on the role of intestinal microflora and gene pathways in the adaptation of BSF larvae to cold stress.

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