文献类型： 外文期刊

作者： Dai, J. J. ¹ ; Wu, C. F. ¹ ; Zhang, D. F. ¹ ; Yin, F. Z. ¹ ; Zhang, T. Y. ¹ ; Liu, D. ¹ ; Wu, H. L. ¹ ; Li, L. L. ¹ ; Yang, ¹ ;

作者机构： 1.Shanghai Acad Agr Sci, Husb & Vet Res Inst, Shanghai 201106, Peoples R China

关键词： maxi-straw

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acrosome morphology rate (NAR) (80.23%). There was no significant difference in the post-thaw motility between different thawing temperatures and corresponding thawing times (p>0.05); the group of 52 degrees C and 25 s gave the highest motility rate (45.00%). As a whole, not only from the motility but also the NAR, thawing at 42 degrees C was better than the other two treatments. In the freezing packages, 5 ml maxi-straw gave a little lower mobility (40%), viability rate (49.58%), plasma membrane integrity rate (53.91%) and NAR (52.65%) than the 0.25 ml straw, but there was no significant difference between the two straw volumes (p>0.05). The IVF capacity of frozen-thawed semen in this experiment was similar to fresh semen. From ultrastructure observation, the main damage to boar spermatozoa after freezing was seen in the acrosome, such as swelling and formation of vesicles, After AI in recipient Shanghai White sows, frozen-thawed semen from 5 ml maxi-straws and pellets produced 72.2% and 80% conception rate and 7.8 and 8 litter sizes, respectively, and there was no significant difference between the 5 ml maxi-straw and the pellet (p>0.05).