文献类型： 外文期刊
作者： Wang, C. 2 ; Liu, Y. 2 ; Zhang, J. 3 ; Wang, H. Y. 1 ; Wu, H. L. 1 ; Gong, S. M. 1 ; He, D. Q. 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China
2.Ningbo Weijia Breeding & Technol Ltd Co, Ningbo, Zhejiang, Peoples R China
3.Southwest Univ, Chongqing 402460, Peoples R China
期刊名称：BRITISH POULTRY SCIENCE （ 影响因子：2.095； 五年影响因子：2.266 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： 1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise the LRP8 gene in goose.2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides) LRP8 gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species.3. Similarly to duck LRP8 gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose.4. Semi-quantitative RT-PCR analysis indicates that the LRP8-1 transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and the LRP8-2 transcript is widely expressed in all examined tissues.5. A total of 7 SNPs were identified in the coding region of the goose LRP8 gene
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