CsMYB Transcription Factors Participate in Jasmonic Acid Signal Transduction in Response to Cold Stress in Tea Plant (Camellia sinensis)
文献类型： 外文期刊
作者： Han, Zhaolan 1 ; Zhang, Chen 1 ; Zhang, Huan 1 ; Duan, Yu 1 ; Zou, Zhongwei 2 ; Zhou, Lin 3 ; Zhu, Xujun 1 ; Fang, Wanping 1 ; Ma, Yuanchun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
2.Wilfrid Laurier Univ, Fac Sci, Dept Biol, Waterloo, ON N2L 3C5, Canada
3.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai 201403, Peoples R China
关键词： Camellia sinensis; MYB TFs; MeJA; cold stress response; interaction
期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）
ISSN：
年卷期： 2022 年 11 卷 21 期
页码：
收录情况： SCI
摘要： Low-temperature stress is an increasing problem for the cultivation of tea (Camellia sinensis), with adverse effects on plant growth and development and subsequent negative impacts on the tea industry. Methyl jasmonate (MeJA), as a plant inducer, can improve the cold-stress tolerance in tea plants. R2R3-MYB transcription factors (TFs) are considered potentially important regulators in the resistance to cold stress in plants. However, the molecular mechanisms, by which MYB TFs via the jasmonic acid pathway respond to cold stress in the tea plant, remain unknown. In this study, physiological and biochemical assays showed that exogenous MeJA application could effectively promote ROS scavenging in the tea plant under cold stress, maintaining the stability of the cell membrane. Sixteen R2R3-MYB TFs genes were identified from the tea plant genome database. Quantitative RT-PCR analysis showed that three CsMYB genes were strongly induced under a combination of MeJA and cold-stress treatment. Subcellular localization assays suggest CsMYB45, CsMYB46, and CsMYB105 localized in the nucleus. Exogenous MeJA treatment enhanced the overexpression of CsMYB45, CsMYB46, and CsMYB105 in E. coli and improved the growth and survival rates of recombinant cells compared to an empty vector under cold stress. Yeast two-hybrid and bimolecular fluorescence complementation experiments confirmed that CsMYB46 and CsMYB105 interacted with CsJAZ3, CsJAZ10, and CsJAZ11 in the nucleus. Taken together, these results highlight that CsMYB45, CsMYB46, and CsMYB105 are not only key components in the cold-stress signal response pathway but also may serve as points of confluence for cold stress and JA signaling pathways. Furthermore, our findings provide new insight into how MYB TFs influence cold tolerance via the jasmonic acid pathway in tea and provide candidate genes for future functional studies and breeding.
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