文献类型： 外文期刊
作者： Zhao, Jiali 1 ; Tan, Zhen 1 ; Wang, Liu 2 ; Lei, Chunyang 1 ; Nie, Zhou 1 ;
作者机构： 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Hunan Prov Key Lab Biomacromol Chem Biol, Changsha 410082, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
期刊名称：CHEMICAL COMMUNICATIONS （ 影响因子：6.222； 五年影响因子：6.008 ）
ISSN： 1359-7345
年卷期： 2021 年 57 卷 57 期
页码：
收录情况： SCI
摘要： Herein, we describe a CRISPR-Cas12a sensing platform activated by a DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD(+), ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR-Cas system into the detection of non-nucleic acid biomolecules.
- 相关文献
作者其他论文 更多>>
-
Precise pathogen quantification by CRISPR-Cas: a sweet but tough nut to crack
作者：Yao, Zhihao;Li, Wanglu;He, Kaiyu;Wang, Hongmei;Xu, Xiahong;Wang, Liu;Yao, Zhihao;Xu, Yan;Wu, Qun;Wang, Liu
关键词：Pathogen; CRISPR-Cas; biosensor; quantification; isothermal amplification
-
Hue-change coupled with CRISPR-Cas12a lateral flow assay for the semiquantitative detection of Salmo salar adulteration
作者：Yuan, Jingrui;Wang, Liu;He, Fang;He, Kaiyu;Wang, Hongmei;Xu, Xiahong;Yuan, Jingrui;Huang, Liang;Wang, Jing;Xu, Xiahong
关键词：Hue change; CRISPR-Cas12a; Lateral flow assay; Quantitative analysis; Salmo salar adulteration
-
Detection of adulteration of non-transgenic soybean oil with transgenic soybean oil by integrating absorption, scattering with fluorescence spectroscopy
作者：He, Xueming;Wang, Meng;You, Jie;Liu, Haowen;Shen, Fei;Li, Peng;Fang, Yong;He, Xueming;Wang, Meng;You, Jie;Liu, Haowen;Shen, Fei;Li, Peng;Fang, Yong;Wang, Liu;Wang, Liu
关键词：Adulteration; transgenic; soybean oil; fluorescence; absorption; scattering
-
Recent advances in spectroscopy and machine learning for non-destructive and real-time detection of mycotoxins in cereals
作者：Mustafa, Ghulam;Wang, Hongmei;Wang, Liu;Yao, Zhihao;Quan, Haoran;He, Kaiyu;Liu, Yuhong;Ali, Maratab
关键词：Mycotoxins; Spectroscopy; Cereals; Machine learning; Non-destructive detection
-
Facilitating crRNA Design by Integrating DNA Interaction Features of CRISPR-Cas12a System
作者：Yao, Zhihao;Xu, Yan;Wu, Qun;Nie, Yao;Yao, Zhihao;Li, Wanglu;He, Kaiyu;Wang, Hongmei;Wang, Liu;Wang, Liu
关键词：activity prediction; CRISPR-Cas12a; deep learning; molecular dynamics simulation; trans-cleavage
-
Molecular Regulation of Cell Wall Structure During Wood Formation in Forest Trees
作者：Yue, Lei;Wei, Xin;Liu, Shuangshuang;Liu, Xing;Wang, Jinwang;Liu, Huizi;Yue, Lei;An, Xia;Gao, Jinghui;Zhao, Jiali;Lin, Renan
关键词：Wood formation; regulatory network; xylem; transcription factors
-
Differentiating Leaf Structures and Physiological Responses to Freezing Stress of Mangrove Kandelia obovata from Different Provenances
作者：Xin, Wenzhen;Liu, Huizi;Liu, Shuangshuang;Yang, Sheng;Wei, Xin;Liu, Xing;Chen, Qiuxia;Xin, Wenzhen;An, Xia;Zhao, Jiali;Lin, Renan
关键词：Kandelia obovata; low temperature; physiological responses; anatomical structure; palisade-to-spongy tissue ratio; SOD
