您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rotary Valve-Assisted Fluidic System Coupling with CRISPR/Cas12a for Fully Integrated Nucleic Acid Detection

文献类型： 外文期刊

作者： Wu, Hui 1 ; Qian, Siwenjie 1 ; Peng, Cheng 2 ; Wang, Xiaofu 2 ; Wang, Tingzhang 3 ; Zhong, Xiaoping 3 ; Chen, Yanju 1 ; Yang, Qunqing 4 ; Xu, Junfeng 2 ; Wu, Jian 1 ;

作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Zhejiang Inst Microbiol, Key Lab Microbiol Technol & Bioinformat Zhejiang, Hangzhou 310012, Peoples R China

4.Zhejiang Police Vocat Acad, Dept Secur & Precaut, Hangzhou 310018, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Site Proc Equipment Agr Prod, Hangzhou 310058, Peoples R China

关键词： nucleic acid detection; rotary valve; Vibrio parahaemolyticus; loop-mediated isothermal amplification; CRISPR/Cas12a

期刊名称：ACS SENSORS （ 影响因子：9.618； 五年影响因子：9.554 ）

ISSN： 2379-3694

年卷期： 2021 年 6 卷 11 期

页码：

收录情况： SCI

摘要： Of late, many nucleic acid analysis platforms have been established, but there is still room for constructing integrated nucleic acid detection systems with high nucleic acid extraction efficiency, low detection cost, and convenient operation. In this work, a simple rotary valve-assisted fluidic chip coupling with CRISPR/Cas12a was established to achieve fully integrated nucleic acid detection. All of the detection reagents were prestored on the fluidic chip. With the aid of the rotary valve and syringe, the liquid flow and stirring can be precisely controlled. The nucleic acid extraction, loop-mediated isothermal amplification (LAMP) reaction, and CRISPR detection could be completed in 80 min. A clean reservoir and an air reservoir on the fluidic chip were designed to effectively remove the remaining ethanol. With Vibrio parahaemolyticus as the targets, the detection sensitivity of the fluidic chip could reach 3.1 x 10(1) copies of target DNA per reaction. A positive sample could be sensitively detected by CRISPR/Cas12a to produce a green fluorescent signal, while a negative sample generated no fluorescent signal. Further, the fluidic chip was successfully applied for detection of spiked shrimp samples, which showed the same detection sensitivity. A great feasibility for real-sample detection was showed by the fluidic chip. The proposed detection platform did not need expensive centrifugal instruments or pumps, which displayed its potential to become a powerful tool for food safety analysis and clinical diagnostics, especially in the resource-limited areas.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台