文献类型： 外文期刊

作者： Cai, Haiming ¹ ; Luo, Shengjun ¹ ; Zhou, Qingfeng ⁶ ; Yan, Zhuanqiang ⁶ ; Liu, Qihong ⁶ ; Kang, Zhen ⁸ ; Liao, Shenquan ¹ ; Li, Juan ¹ ; Lv, Minna ¹ ; Lin, Xuhui ¹ ; Hu, Junjing ¹ ; Yu, Shuilan ⁶ ; Zhang, Jianfei ¹ ; Qi, Nanshan ¹ ; Sun, Mingfei ¹ ;

作者机构： 1.Guangdong Lab Lingnan Modern Agr Sci & Technol, Zhaoqing Maoming Branch Ctr, Guangzhou 510640, Guangdong, Peoples R China

2.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Guangdong, Peoples R China

3.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Guangdong, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Avian Influenza & Other Major Poultry Dis, Guangzhou 510640, Guangdong, Peoples R China

5.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China

6.Wens Foodstuffs Grp Co Ltd, Wens Grp Acad, Yufun 527400, Guangdong, Peoples R China

7.Jiangsu HFQ Biotechnol Co Ltd, Haimen, Jiangsu, Peoples R China

8.Qingdao Vland Biotech Grp Co Ltd, Qingdao, Shandong, Peoples R China

关键词： Bacillus subtilis; coccidiosis vaccine; growth performance; histopathology; Intestinal microbiota

期刊名称：POULTRY SCIENCE （ 影响因子：4.014； 五年影响因子：4.192 ）

ISSN： 0032-5791

年卷期： 2022 年 101 卷 11 期

页码：

收录情况： SCI

摘要： Avian coccidiosis is the most serious par-asitic disease in the poultry industry. Therefore, the aim of the current study was to explore the effects of B. sub-tilis and live coccidiosis vaccine alone or in combination on the production performance and anticoccidiosis, as well as the dynamic changes of intestinal microbial com-munity. Nine hundred ninty Mahuang chickens were randomized into 4 preimmune groups including control group, coccidiosis vaccine immunization group; B. subti-lis administration group and a group that was adminis-trated a combination of live coccidiosis vaccine and B. subtilis group. Intestinal mucosal scraps collected from all these experimental groups at the age of 8 d and 15 d for microbial community 16S rRNA gene sequencing. At the age of 25 d, 30 broilers from each preimmune group were randomly assigned to a subgroup infected with Eimeria spp. and renamed as CI, V-CI BS-CI, and VBS-CI group. The production performance was monitored at the age of 25 d, 35 d, 45 d, and 55 d for the rest broilers from each pre-immune group. Otherwise, in the Eimeria spp. challenge stage, intestinal mucosal scraps collected for microbial community sequencing, while duodenum, jejunum, and cecum collected for pathologi-cal examination after sacrifice at the age of 32 d. In addi-tion, the oocysts per gram of feces (OPG) and intestinal lesion score of broilers after Eimeria spp. challenge were also counted. Overall, the probiotics and coccidiosis vac-cine resulted in the significantly improvement of the pro-duction performance. Otherwise, the intestinal lesion score and OPG after Eimeria spp. infection was signifi-cantly decreased in the VBS-CI group (P < 0.05). More-over, these protective effects may also be closely related to genus such as Romboutsia, Blautia, and Butyricococ-cus, as well as microbiota functions like the quorum sensing pathway. According to these results, a combina-tion of B. subtilis and coccidiosis vaccines can improve performance and provide additional protection against Eimeria spp. infection.