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Insights into heat response mechanisms in Clematis species: physiological analysis, expression profiles and function verification

文献类型： 外文期刊

作者： Zhang, Hao 1 ; Jiang, Changhua 1 ; Wang, Rui 2 ; Zhang, Long 2 ; Gai, Ruonan 2 ; Peng, Siyuan 2 ; Zhang, Yi 2 ; Mao, Chan 1 ;

作者机构： 1.Shanghai Urban Plant Resources Dev & Applicat Eng, Shanghai Bot Garden, Shanghai 200231, Peoples R China

2.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China

3.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： Clematis; Heat stress; Transcriptome; HSFs; HSPs; VIGS

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2021 年 106 卷 6 期

页码：

收录情况： SCI

摘要： Key message Our results provide insights into heat response mechanisms among Clematis species. Overexpressing CvHSFA2 enhanced the heat resistance of yeast and silencing NbHSFA2 reduced the heat resistance of tobacco. Clematis species are commonly grown in western and Japanese gardens. Heat stress can inhibit many physiological processes mediating plant growth and development. The mechanism regulating responses to heat has been well characterized in Arabidopsis thaliana and some crops, but not in horticultural plants, including Clematis species. In this study, we found that Clematis alpina 'Stolwijk Gold' was heat-sensitive whereas Clematis vitalba and Clematis viticella 'Polish Spirit' were heat-tolerant based on the physiological analyses in heat stress. Transcriptomic profiling identified a set of heat tolerance-related genes (HTGs). Consistent with the observed phenotype in heat stress, 41.43% of the differentially expressed HTGs between heat treatment and control were down-regulated in heat-sensitive cultivar Stolwijk Gold, but only 9.80% and 20.79% of the differentially expressed HTGs in heat resistant C. vitalba and Polish Spirit, respectively. Co-expression network, protein-protein interaction network and phylogenetic analysis revealed that the genes encoding heat shock transcription factors (HSFs) and heat shock proteins (HSPs) may played an essential role in Clematis resistance to heat stress. Two clades of heat-induced CvHSFs were further identified by phylogenetic tree, motif analysis and qRT-PCR. Ultimately, we proposed that overexpressing CvHSFA2-2 could endow yeast with high temperature resistance and silencing its homologous gene NbHSFA2 reduced the heat resistance of tobacco. This study provides first insights into the diversity of the heat response mechanisms among Clematis species.

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