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Sucrose phosphate synthase 8 is required for the remobilization of carbon reserves in rice stems during grain filling

文献类型： 外文期刊

作者： Ma, Mingyang 1 ; Zhu, Tong 1 ; Cheng, Xiuyue 1 ; Li, Mengyu 1 ; Yuan, Guoliang 2 ; Li, Changbao 2 ; Zhang, Aihong 1 ; Lu, Congming 1 ; Fang, Ying 1 ; Zhang, Yi 1 ;

作者机构： 1.Shandong Agr Univ, Coll Life Sci, Natl Key Lab Wheat Improvement, Tai An 271018, Shandong, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China

关键词： Crop yield; grain filling; non-structural carbohydrate; rice; starch; stems; sucrose phosphate synthase

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Carbon reserve remobilization in stems is closely related to rice grain filling. Sucrose phosphate synthase (SPS) is highly associated with carbon reserve remobilization. In this study, we investigated the expression pattern of SPS genes in various rice tissues, and found that SPS8 is the major SPS isoform in rice stems during the grain-filling stage. We then constructed sps8 mutants using the CRISPR/Cas9 system. The SPS activity of the sps8 mutants was markedly reduced in the stems. In addition, the sps8 mutants exhibited significant starch accumulation in stems. C-14-labelling experiments revealed that the remobilization of non-structural carbohydrates from rice stems to grains was impaired in the sps8 mutants. In the sps8 mutants, grain filling was delayed and yield decreased by 15% due to a reduced percentage of ripened grains. RNA sequencing and quantitative PCR analyses indicated that the genes involved in starch synthesis and degradation were up-regulated in the sps8 mutant stems. In addition, the activity of the enzymes involved in starch synthesis and degradation was increased in the sps8 stems. These results demonstrate that SPS8 is required for carbon reserve remobilization from rice stems to grains, and that its absence may enhance 'futile cycles' of starch synthesis and degradation in rice stems.

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