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Structure, Intracellular Localisation and Expression Analysis of Sucrose Nonfermenting-Related Kinase ShSnRK1 alpha in Sugarcane

文献类型： 外文期刊

作者： Zhao, Ting-ting 1 ; Wang, Jun-gang 1 ; Wang, Wen-zhi 1 ; Feng, Cui-lian 1 ; Feng, Xiao-yan 1 ; Shen, Lin-bo 1 ; Zhang, Shu-zhen 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Crops Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, 4 Coll Rd, Haikou 571101, Hainan, Peoples R China

2.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan, Haikou, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Sugarcane Res Ctr, Haikou, Hainan, Peoples R China

关键词： ShSnRK1 alpha; Intracellular localisation; Expression analysis; Sucrose accumulation; Sugarcane

期刊名称：SUGAR TECH （ 影响因子：1.872； 五年影响因子：1.873 ）

ISSN： 0972-1525

年卷期：

页码：

收录情况： SCI

摘要： Sucrose nonfermenting-related kinase 1 (SnRK1) function as a key regulator in sensing sucrose-Tre6P (trehalose 6-phosphate) signalling and negatively regulating sucrose homeostasis and content in plants. To clarify the potential functions of SnRK1 in sugarcane, we isolated a SnRK1 catalytic a subunit gene ShSnRK1 a from sugarcane. The structure conservation, intracellular localisation and gene expression of ShSnRK1 alpha were analysed. Results showed that ShSnRK1 alpha had a 1527 bp open reading frame encoding 509 amino acids. The protein sequence of ShSnRK1 alpha contained three conserved domains, including an N-terminal catalytic protein-kinase domain, a middle-plant-specific ubiquitin associated (UBA) domain and a C-terminal regulatory (alpha CTD) domain. The N-terminal protein-kinase domain of ShSnRK1 alpha was highly conserved in SnRK1 alpha s from monocot and dicot plant species. The UBA and alpha CTD domains of ShSnRK1 alpha had sugarcane specificity. ShSnRK1 alpha shared high identity with SnRK1 as from sorghum, maize and rice. Intracellular localisation analysis showed that ShSnRK1 alpha localised in the nucleus and cytoplasm regions of rice mesophyll protoplasts. Expression analysis showed that ShSnRK1 alpha was constitutively expressed in sugarcane leaves and stem internodes. ShSnRK1 alpha was more highly expressed in sucrose rapid-accumulation maturing internodes than in immature and mature internodes. Meanwhile, ShSnRK1 alpha was expressed lower in leaves and sucrose accumulating internodes of high-sugar-content sugarcane plants than that of low-sugar-content sugarcane plants. Thus, ShSnRK1 alpha may play an important role on regulating sucrose homeostasis and accumulation in sugarcane. This study provided a target to clarify the sugar-accumulation regulating mechanism of sugarcane and improve its sugar content.

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