Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus
文献类型: 外文期刊
作者: Zhang, Yu-liang 1 ; Zhang, Shu-zhen 1 ; Kulye, Mahesh 2 ; Wu, Su-ran 1 ; Yu, Nai-tong 1 ; Wang, Jian-hua 1 ; Zeng, Hon 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, Minist Agr, Key Lab Integrated Pest Management Crops, Beijing 10
关键词: bacteria-expressed dsRNA;bioassay;ecdysteroid signaling;phylogenetic analysis;quantitative real-time PCR;RNA interference
期刊名称:JOURNAL OF INSECT SCIENCE ( 影响因子:1.857; 五年影响因子:1.904 )
ISSN: 1536-2442
年卷期: 2012 年 12 卷
页码:
收录情况: SCI
摘要: RNA interference (RNAi) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. Development of reliable methods for production and delivery of double-stranded RNA (dsRNA) is the major challenge for efficient pest control. In this study, Chilo infuscatellus Snellen (Crambidae: Lepidoptera) was fed with CiHR3 dsRNA expressed in bacteria or synthesized in vitro. The dsRNA ingested by C. infuscatellus successfully triggered silencing of the molt-regulating transcription factor CiHR3, an important gene for insect growth and development, and caused significant abnormalities and weight loss in insects within seven days of treatment. This study is an ideal example of feeding-based RNAi mediated by dsRNA expressed in bacteria or synthesized in vitro. The results also suggested that feeding-based RNA interference is a potential method for the management of C. infuscatellus.
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