Immunodominant membrane protein (Imp) promotes the transmission of wheat blue dwarf (WBD) phytoplasma by directly interacting with alpha-tubulin in leafhoppers
文献类型： 外文期刊
作者： Ding, Lei 1 ; Lu, Wenjing 1 ; Yang, Yanjun 1 ; Zhong, Qinrong 1 ; Zhou, Tong 2 ; Wang, Guiling 2 ; Song, Chengyan 2 ; Ma, Wendong 2 ; Chen, Wei 3 ; Wu, Yunfeng 1 ;
作者机构： 1.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Key Lab Crop Pest Integrated Pest Management Loes, Yangling 712100, Xianyang, Peoples R China
2.Heilongjiang Acad Agr Sci, Rice Res Inst, Jiamusi 154026, Peoples R China
3.Shanxi Normal Univ, Coll Life Sci, Linfen 041000, Shanxi, Peoples R China
关键词： Immunodominant membrane protein (Imp); Transmission mechanism; alpha-Tubulin; Psammotettix striatus; Wheat blue dwarf (WBD) phytoplasma
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.224； 五年影响因子：2.262 ）
ISSN： 0929-1873
年卷期： 2022 年 162 卷 2 期
页码：
收录情况： SCI
摘要： Phytoplasmas are important plant pathogens that affect crops, bushes, and trees. Wheat blue dwarf (WBD) phytoplasma is transmitted by the leafhopper Psammotettix striatus, with infection resulting in severe losses in wheat yield in Northwest China. Immunodominant membrane protein (Imp) potentially plays a crucial role in phytoplasma transmission, although the mechanisms of WBD phytoplasma transmission has not yet been elucidated. In this study, the interaction between alpha-tubulin and Imp was confirmed by a split-ubiquitin membrane yeast two-hybrid assay, and co-immunoprecipitation (co-IP) assay. Co-expression of mNeonGreen-Imp and mScarlet-alpha-tubulin fusion proteins in the baculovirus expression vector system (BEVS) showed that Imp colocalizes with alpha-tubulin in the cytoplasm of Spodoptera frugiperda (Sf9) cells near the cell membrane. Suppression of alpha-tubulin transcription by RNA interference (RNAi) decreased the transmission efficiency of the WBD phytoplasma. Results of this study show that Imp employs alpha-tubulin to promote transmission of WBD phytoplasma via leafhopper. To our knowledge, this is the first report that describes the molecular interactions between the WBD phytoplasma and leafhopper. These results may help elucidate the mechanisms governing transmission of phytoplasma by its insect vector and provide a candidate target gene for controlling spread of WBD phytoplasma in the field using the RNAi approach.
- 相关文献
作者其他论文 更多>>
-
Pedigree genome data of an early-matured Geng/japonica glutinous rice mega variety Longgeng 57
作者：Lei, Yuanbao;Zhang, Yunjiang;Ma, Wendong;Lei, Yuanbao;Xu, Linyun;Zhou, Ziqi;Yang, Dechen;Zhang, Fan;Zhou, Yongli;Zhao, Xiuqin;Wang, Wensheng;Li, Zhikang;Xu, Jianlong;Zheng, Tianqing;Lei, Yuanbao;Liu, Bailong;Li, Jie;Quan, Pengyu;Quan, Guangjun;Faruquee, Muhiuddin;Yang, Dechen;Wang, Wensheng;Xu, Jianlong;Zheng, Tianqing;Xu, Jianlong
关键词：
-
Screening and analysis of candidate genes conferring alkalinity tolerance in rice (Oryza sativa L.) at the bud burst stage based on QTL-seq and RNA-seq
作者：Wang, Jiangxu;Sun, Shichen;Jiang, Hui;Chen, Lei;Lei, Lei;Liu, Kai;Wang, Jiangxu;Sun, Shichen;Jiang, Hui;Chen, Lei;Lei, Lei;Liu, Kai;Wang, Jiangxu;Bian, Jingyang;Liu, Linshuai;Gao, Shiwei;Liu, Qing;Feng, Yanjiang;Shan, Lili;Guo, Junxiang;Wang, Guiling
关键词：Alkalinity tolerance; Bud burst; Candidate genes; Cropland; Oryza sativa L; QTL-seq; Rice; RNA-seq; Soil salinization
-
Comparative Metabolome Profiling for Revealing the Effects of Different Cooking Methods on Glutinous Rice Longjing57 (Oryza sativa L. var. Glutinosa)
作者：Guo, Zhenhua;Liu, Chuanxue;Zhang, Yunjiang;Wang, Linan;Feng, Yanjiang;Pan, Guojun;Ma, Wendong;Guo, Zhenhua;Cai, Lijun;Liu, Hao
关键词：glutinous rice; cooking; widely targeted metabolome; multivariate analysis
-
Transgenic early japonica rice: Integration and expression characterization of stem borer resistance Bt gene
作者：Hu, Yueting;Tian, Chongbing;Feng, Yanjiang;Ma, Wendong;Zhang, Yunjiang;Yang, Qing;Zhang, Xirui
关键词：Transgenic early japonica rice; cry1C*gene; cry2A*gene; Southern blot; Bt protein; Expression characteristics
-
RICE ADAPTS TO ALKALI STRESS BY MICROSTRUCTURAL CHANGES
作者：Zhao, Haixin;Du, Xiaodong;Chen, Shuqiang;Yang, Limin;Huang, Xiaoqun;Ma, Wendong;Cai, Yongsheng;Pan, Guojun;Peng, Feng;Zheng, Haiyan
关键词：Alkalin stress; Rice variety; Cell microstructure; Response analysis
-
Molecular evidence to the day length in regulating the short shoot phenomenon during summer in roses (Rosa sp.)
作者：Wang, Li;Jiang, Wan;Zhang, Wuhua;Chen, Weitong;Mo, Yu;Zhou, Tong;Zhang, Jinzhu;Che, Daidi;Wang, Li
关键词：Day length; short shoot phenomenon in summer; abscisic acid; auxin
-
Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties
作者：Guo, Zhenhua;Ma, Wendong;Wang, Linan;Feng, Yanjiang;Liu, Chuanxue;Pan, Guojun;Guo, Zhenhua;Guo, Tao;Cai, Lijun;Liu, Junliang;Liu, Hao;Ma, Bo
关键词：Rice; Cold stress; Reproductive stage; Transcriptome analysis; Plant hormone
