Optimization of sodium dedecyl sulfate (SDS) addition coupled with adenosine triphosphate (ATP) regeneration for glutathione overproduction in high density cultivation of Candida utilis
文献类型： 外文期刊
作者： Liang, Guobin 1 ; Mo, Yiwei 2 ; Du, Guocheng 3 ;
作者机构： 1.Jiangsu Teachers Univ Technol, Sch Chem & Environm Engn, Changzhou 213001, Peoples R China
2.Chinese Acad Trop Agr Sci, S Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China
3.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China
关键词： Glutathione;Sodium dedecyl sulfate (SDS);Adenosine triphosphate (ATP) regeneration;Glucose feeding;Candida utilis
期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.493； 五年影响因子：3.699 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： In the previous study, glutathione production was elevated by adding precursor amino acids and adenosine triphosphate (ATP) in high cell density cultivation of Candida utilis. Furthermore, in the present research, glutathione production was further improved by optimizing sodium dedecyl sulfate (SDS) addition coupled with ATP regeneration. Results indicated that with 2g/l ATP added at 60 h and 0.8 g/l SDS added repeatedly at 60,63,66 and 69 h, final glutathione yield reached 2286 mg/l after 72 h cultivation. Moreover, by applying the novel strategies of regenerating ATP by feeding glucose at 6g/(lh) from 60 to 72 h coupled with impulse SDS treatments, a total glutathione yield of 2485 mg/l was achieved at 72 h, which was 8.7% higher than with addition of ATP and SDS, suggesting application of the proposed strategies as being feasible for glutathione overproduction on industrial scales.
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