Comparative genomic and transcriptome analysis of Bacillus velezensis CL-4 fermented corn germ meal
文献类型： 外文期刊
作者： Chen, Long 1 ; Qu, Zihui 1 ; Yu, Wei 1 ; Zheng, Lin 1 ; Qiao, Haixin 3 ; Wang, Dan 1 ; Wei, Bingdong 1 ; Zhao, Zijian 2 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Nutr & Feed, 186 Dong Xinghua St, Gongzhuling 136100, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Agrofood Technol, 1366 Cai Yu St, Changchun 130033, Jilin, Peoples R China
3.Jilin Intellectual Property Protect Ctr, Informat Applicat Dept, Changchun 130000, Peoples R China
关键词： B; velezensis CL-4; Comparative genomics; Transcriptome; Lignocellulose-degrading enzymes; Corn germ meal
期刊名称：AMB EXPRESS （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN： 2191-0855
年卷期： 2023 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Bacillus, an excellent organic-degrading agent, can degrade lignocellulose. Notably, some B. velezensis strains encode lignocellulases. However, their ability to degrade lignocellulose in fermented feed is not much appreciated. This study performed a comparative genomic analysis of twenty-three B. velezensis strains to find common carbohydrate-active enzymes (CAZymes) encoding genes and evaluated their potential to degrade lignocellulose. The comparative genomic and CAZyme database-based analyses identified several potential CAZymes genes that degrade cellulose (GH1, GH4, GH5, GH13, GH16, GH32, PL1, and PL9), hemicellulose (GH11, GH26, GH43, GH51, and CE3) and lignin (AA4, AA6, AA7, and AA10). Furthermore, Illumina RNA-seq transcriptome analysis revealed the expression of more than 1794 genes in B. velezensis CL-4 fermented corn germ meal at 48 h (FCGM 48 h). Gene ontology analysis of expressed genes revealed their enrichment in hydrolase activity (breaking the glycosyl bonds during carbohydrate metabolism), indicating the upregulation of CAZymes. In total, 58 differentially upregulated CAZymes-encoding genes were identified in FCGM 48 h compared to FCGM 0 h. The upregulated CAZymes-encoding genes were related to cellulose (6-phospho-beta-galactosidase and 6-phospho-alpha-glucosidase), starch (alpha-glucosidase and alpha-amylase), pectin (pectin lyase), and hemicellulose (arabinan endo-1,5-alpha-L-arabinosidase, xylan 1,4-beta-xylosidase, alpha-N-arabinofuranosidase, and acetyl xylan esterase). Importantly, arabinoxylan degradation mainly occurred in FCGM 48 h, followed by partial degradation of cellulose, pectin, and starch. This study can support the development of enzymatic cocktails for the solid-state fermented feed (SFF).
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