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Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366

文献类型： 外文期刊

作者： Liu, Yong-jie 1 ; Li, Dan 1 ; Gong, Jie 1 ; Wang, Yong-bo 1 ; Chen, Zhao-bo 1 ; Pang, Bin-shuang 1 ; Chen, Xian-chao 1 ; Gao, Jian-gang 1 ; Yang, Wei-bing 1 ; Zhang, Feng-ting 1 ; Tang, Yi-miao 1 ; Zhao, Chang-ping 1 ; Gao, Shi-qing 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Engn Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China

2.Municipal Key Lab Mol Genet Hybrid Wheat, Beijing 100097, Peoples R China

关键词： Triticum aestivum L; Pollen; Temperature-sensitive genic male sterility (TGMS); Transcriptome; DNA methylation

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 degrees C with 12 h of daylight) but fertile under normal temperature (20 degrees C with 12 h of daylight). Results During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division. Conclusions These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility.

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