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Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep TBXT Heterozygotes

文献类型： 外文期刊

作者： Yang, Guang 1 ; Wang, Caiyun 2 ; Su, Hong 2 ; Wang, Daqing 2 ; Dou, Aolie 2 ; Chen, Lu 2 ; Ma, Teng 2 ; Liu, Moning 2 ; Su, Jie 2 ; Xu, Xiaojing 2 ; Yang, Yanyan 3 ; He, Tingyi 3 ; Li, Xihe 1 ; Song, Yongli 1 ; Cao, Guifang 2 ;

作者机构： 1.Inner Mongolia Univ, Sch Life Sci, Hohhot 010021, Peoples R China

2.Inner Mongolia Agr Univ, Sch Vet Med, Hohhot 010010, Peoples R China

3.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Anim Husb, Hohhot 010031, Peoples R China

关键词： short-tailed trait; sheep; TBXT; HRM

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 6 期

页码：

收录情况： SCI

摘要： Simple Summary TBXT (c.333G > C; c.334G > T) has been identified as a molecular genetic marker in short-tailed sheep. This paper describes a high-resolution melting (HRM) analysis using unlabeled probes and asymmetric PCR for the detection of genetic variants of TBXT in short-tailed sheep populations. The detection results of this method are consistent with those of Sanger sequencing and can help farmers with marker-assisted breeding. The short-tailed phenotype has long been considered one of the best traits for population genetic improvement in sheep breeding. In short-tailed sheep, not only is tail fat eliminated but also the pubic area is exposed due to the lack of a tail covering, giving them an advantage in reproduction. Recent studies have shown that two linked mutations in sheep TBXT at nucleotides 333 and 334 are associated with the short-tailed phenotype. In the population of short-tailed sheep, several heterozygous mutants of this gene are found. In our research, we used high-resolution melting (HRM) to identify homozygous and heterozygous genotypes in a flock of short-tailed sheep and compared the results with those of Sanger sequencing, which were identical. This demonstrates that our established HRM method, a rapid and inexpensive genotyping method, can be used to identify homozygous and heterozygous individuals in short-tailed sheep flocks.

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