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Non-separated microspores 1 controls male meiotic callose deposition at the cell plate in rice

文献类型： 外文期刊

作者： Chen, Haiyuan 1 ; Zhang, Suobing 1 ; Tang, Weijie 1 ; Tang, Jun 1 ; Lin, Jing 1 ; Fang, Xianwen 1 ; Zhang, Yunhui 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

2.Zhongshan Biol Breeding Lab, Nanjing 210014, Jiangsu, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Callose synthase; Microspore separation; Exine; Sporopollenin; Rice

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2024 年 12 卷 6 期

页码：

收录情况： SCI

摘要： In flowering plants, callose (/3-1,3-glucan) plays a vital role in pollen development, especially in the separation and development of microspores. However, the molecular mechanism of callose deposition during rice pollen development remains unclear. In this study, we isolated and characterized a novel rice pollen defective mutant, non-separated microspores 1 (nsm1), which produced "dyad" or "tetrad" pollen grains. Cytological analysis indicated disrupted interstitial callose deposition at the cell plate of dyads and tetrads in nsm1 pollens. This disruption caused sporopollenin to be massively deposited outside of the junction where the interstitial callose wall connected with the peripheral callose wall, or unevenly distributed on the interstitial pollen primexine at the late meiosis stage. Consequently, an excess tectum-like layer was formed outside of the junction, connecting with the tectum of two microspores during later developmental stages, which prevented the separation of microspores. Additionally, in the linkage area, the tectum of two microspores gradually fused or degenerated, resulting in a decreased contact area between microspores and the anther locule. Therefore, the defect in callose deposition resulted in unsuccessful separation of microspores, abnormal deposition of pollen exine, and also affected the accumulation of materials in microspores, resulting in pollen semi-sterility. NSM1, encoding a callose synthase located in the Golgi body, is ubiquitously expressed in anthers with its peak expression at the young microspore stage. The in vitro enzyme activity assay confirmed that NSM1 possesses callose synthase activity, and the enzyme activity in the nsm1 mutants was significantly reduced. Phylogenetic analysis indicated that NSM1 and its orthologs play a highly conserved role in callose biosynthesis among plant species. Taken together, we propose that NSM1 plays an essential role in male meiotic callose synthesis and later pollen wall development. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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