CRISPR/Cas-SERS Sensing Platforms: A Frontier Technology for Next-Generation Fast, Low-Cost, Ultra-Micro Biosample Detection
文献类型： 外文期刊
作者： Bao, Chengxin 1 ; Liu, Xiangguo 2 ; Liang, Chongyang 3 ; Xu, Shuping 1 ;
作者机构： 1.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun, Peoples R China
2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun, Peoples R China
3.Jilin Univ, Inst Frontier Med Sci, Changchun, Peoples R China
4.Jilin Univ, Coll Chem, Ctr Supramol Chem Biol, Changchun, Peoples R China
关键词：
CRISPR/Cas detection; nucleic acid analysis; SERS detection;
期刊名称：JOURNAL OF RAMAN SPECTROSCOPY （ 影响因子：1.9； 五年影响因子：2.5 ）
ISSN： 0377-0486
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Researchers have long been interested in nucleic acid detection technology. Surface-enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost-effectiveness, and rapidity compared to conventional nucleic acid analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest in nucleic acid analysis due to its precise identification and isothermal advantages. Integrating the CRISPR/Cas system's specific identification capabilities with the high-sensitivity fingerprinting properties of SERS offers a sensitive, ultra-low volume, rapid, and straightforward method for detecting new nucleic acid modalities. This review delineates the components and characteristics of the CRISPR/Cas system, encompassing three Cas proteins (Cas9, Cas12, and Cas13) and detection technologies derived from CRISPR/Cas, namely, high-sensitivity enzymatic reporter unlocking (SHERLOCK) and DNA endonuclease-targeted CRISPR trans reporters (DETECTR). Advancements in SERS and CRISPR/Cas-SERS-based nucleic acid assays were emphasized, encompassing traditional SERS and CRISPR/Cas-SERS-based nucleic acid and non-nucleic acid tests. Examples encompass microfluidics/microdroplet-based CRISPR/Cas-SERS and non-amplified detection based on CRISPR/Cas-SERS, and so on.
- 相关文献
作者其他论文 更多>>
-
Powerful combination: a genome editing system to improve efficiency of breeding inducer and haploid sorting in maize
作者：Xia, Hanchao;Yin, Yuejia;Zhang, Chuang;Chen, Ziqi;Liu, Xiangguo;Xia, Hanchao;Zhang, Di;Zhao, Rengui;Liu, Xiangguo;Qu, Yanzhi;Jiang, Shurong;Wang, Xinqi;Xu, Jieting
关键词：haploid; MoDsRed2; inducer line; genome-editing; Zea mays
-
CRISPR/dCas9-assisted SERS sensing for transgenic crops based on two-factor verification of 35S promoter and Nos terminator
作者：Su, Ailing;Xu, Weiqing;Xu, Shuping;Chen, Ziqi;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping;Xu, Shuping
关键词：CRISPR/dCas9; SERS; Gene detection; Transgenic crop identification; Multiplex detection
-
LcTprxII Overexpression Enhances Physiological and Biochemical Effects in Maize Under Alkaline (Na2CO3) Stress
作者：Michael, David Pitia Julius;Lu, Jainyu;Rehman, Faiz Ur;Temitope, Aroge;Qian, Buxuan;Liu, Xiangguo;Qi, Xin;Sun, Ruidong;Chen, Ziqi;Zhang, Jian;Liu, Qing;Wei, Xuancheng;Xia, Hanchao;Han, Jiarui;Liu, Xiangguo;Chen, Ziqi;Yin, Yuejia;Jiang, Long
关键词：LcTprxII; alkaline stress; type II peroxiredoxin; transcriptome; hormone regulation
-
Amplification-free detection of genetically modified crops via spatial confinement CHA/HCR-aided surface-enhanced resonance Raman spectroscopy
作者：Bao, Chengxin;Wang, Huimin;Liu, Lin;Xu, Shuping;Li, Feiwu;He, Yuxuan;Yin, Yuejia;Liu, Xiangguo;Liu, Lin;Chang, Jingjing;Ding, Lan;Xu, Shuping;Xu, Shuping
关键词：Amplification-free; CHA; HCR; Spatial confinement; SERS
-
Isolation and Functional Analysis of Na+/H+ Antiporter Gene (LcNHX1) from Leymus chinensis
作者：Sun, Chuanbo;Zhang, Chuang;Yin, Yuejia;Mu, Shujing;Liu, Qing;Wang, Ziyu;Liu, Yang;Zhang, Chunxiao;Liu, Xiangguo;Zhang, Yan;Guo, Jia;Wang, Ying
关键词：NHX1; Leymus chinensis; Saline-alkali tolerance; Candidate gene
-
Gene editing of ZmGA20ox3 improves plant architecture and drought tolerance in maize
作者：Liu, Yang;Chen, Ziqi;Zhang, Chuang;Guo, Jia;Liu, Qing;Yin, Yuejia;Hu, Yang;Xia, Hanchao;Li, Bingyang;Li, Yidan;Liu, Xiangguo;Xia, Hanchao;Sun, Xiaopeng;Sun, Xiaopeng
关键词：Maize; Drought stress; Gibberellin; Yield; GA20-oxidase
-
Sensitive detection of genetically modified maize based on a CRISPR/Cas12a system
作者：Wang, Huimin;Su, Ailing;Xu, Shuping;Chang, Jingjing;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping;Xu, Shuping
关键词：
