文献类型： 外文期刊

作者： Michael, David Pitia Julius ¹ ; Liu, Qing ² ; Yin, Yuejia ³ ; Wei, Xuancheng ² ; Lu, Jainyu ¹ ; Rehman, Faiz Ur ¹ ; Temitope, Aroge ¹ ; Qian, Buxuan ¹ ; Xia, Hanchao ² ; Han, Jiarui ² ; Liu, Xiangguo ¹ ; Jiang, Long ⁴ ; Qi, Xin ¹ ; Sun, Ruidong ¹ ; Chen, Ziqi ¹ ; Zhang, Jian ¹ ;

作者机构： 1.Jilin Agr Univ, Fac Agron, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Northeast Agr Res Ctr China, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China

3.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Northeast Agr Res Ctr China, Changchun 130033, Peoples R China

4.Jilin Agr Sci & Technol Univ, Coll Agron, Jilin 132101, Peoples R China

关键词： LcTprxII; alkaline stress; type II peroxiredoxin; transcriptome; hormone regulation

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Alkaline stress limits crop productivity by causing osmotic and oxidative damage. This study investigated the new gene LcTprxII, a type II peroxiredoxin encoded by Leymus chinensis, and its role in enhancing alkaline stress tolerance in transgenic maize. The gene was cloned, overexpressed, and characterized using RT-PCR, phylogenetic analysis, and motif identification. Transgenic maize lines were generated via Agrobacterium-mediated transformation and subjected to physiological, biochemical, and transcriptomic analyses under alkaline stress. Under alkaline stress, the results revealed that LcTprxII overexpression significantly preserved chlorophyll content, mitigated oxidative damage, and maintained growth compared to wild-type plants, as evidenced by elevated activities of antioxidant enzymes (APX, CAT, SOD, and POD) and reduced malondialdehyde (MDA) content. Transcriptomic profiling identified 3733 differentially expressed genes and the upregulation of ABA and MAPK signaling pathways, highlighting the role of these genes in stress signaling and metabolic adaptation. Hormonal analysis indicated reduced ABA and increased GA levels in the transgenic lines. This study identified WRKY, bHLH, and MYB transcription factors as key regulators activated under alkaline stress, contributing to transcriptional regulation in transgenic maize. Field trials confirmed the agronomic potential of LcTprxII-overexpressing maize, with yield maintained under alkaline conditions. The present study revealed that LcTprxII enhances antioxidant defenses and stress signaling, which trigger tolerance to abiotic stress. Future studies should explore the long-term effects on growth, yield, and molecular interactions under diverse environmental conditions.