Transcriptome analysis reveals the mechanism of alkalinity exposure on spleen oxidative stress, inflammation and immune function of Luciobarbus capito
文献类型： 外文期刊
作者： Shang, Xinchi 1 ; Geng, Longwu 1 ; Yang, Jian 1 ; Zhang, Yuting 1 ; Xu, Wei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Rd 43 Songfa, Harbin 150070, Peoples R China
2.Key Lab Cold Water Fish Germplasm Resources & Mul, Harbin 150070, Heilongjiang, Peoples R China
关键词： Transcriptome; Alkalinity; Oxidative stress; Inflammation; Immune
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）
ISSN： 0147-6513
年卷期： 2021 年 225 卷
页码：
收录情况： SCI
摘要： Saline-alkali land is distributed all over the world, and it affects the economic development of fisheries. The alkalinity in water is related to the accumulation of carbonate, so the is generally higher. To understand how alkalinity impacts the immune response in Luciobarbus capito, we performed transcriptomic profiles for spleen, the immune organ of Luciobarbus capito which were underwent alkalinity exposure. Totally there are 47,727,954, 53,987,820 and 51,398,546 high quality clean reads obtained from the control groups, and 46,996,982, 49,650,460 and 45,964,986 clean reads from the alkalinity exposure groups. Among them, 611 genes were differently expressed, including 534 upregulated and 77 down-regulated genes. The identified genes were enriched using databases of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). It was found that differentially expressed genes in Luciobarbus capito spleen tissue were enriched into 14 GO pathways, and differentially expressed genes in Luciobarbus capito spleen were enriched into 25 corresponding KEGG pathways under alkalinity stress. Inflammation and immune function genes and pathways were identified and validated by quantitative real-time RT-PCR. Our results showed that alkalinity exposure leads to inflammation and immunoregulation in spleen of Luciobarbus capito. These results provide new insights for unveiling the biological effects of alkalinity in Luciobarbus capito.
- 相关文献
作者其他论文 更多>>
-
Identification of "Bathed" Chinese Mitten Crabs (Eriocheir sinensis) Using Geometric Morphological Analysis of the Carapace
作者：Xu, Yiqian;Liu, Hongbo;Yang, Jian;Xue, Junren;Liu, Hongbo;Jiang, Tao;Chen, Xiubao;Yang, Jian
关键词：Eriocheir sinensis; landmark; geometric morphometrics; "introduced"/"bathed" crab; discriminant analysis; aquaculture
-
Otolith biogeochemistry reveals possible impacts of extreme climate events on population connectivity of a highly migratory fish, Japanese Spanish mackerel Scomberomorus niphonius
作者：Pan, Xindong;Tian, Yongjun;Pan, Xindong;Tian, Yongjun;Pan, Xindong;Tian, Yongjun;Pan, Xindong;Chen, Yong;Jiang, Tao;Yang, Jian
关键词：Population connectivity; ENSO; Otolith biogeochemistry; Migration; Scomberomorus niphonius
-
Reconstruction of Historical Metal Backgrounds in Lacustrine Environments of China Using an Anodonta woodiana "Specimen Bank"
作者：Yan, Mingjun;Yang, Jian;Chen, Xiubao;Xue, Junren;Liu, Hongbo;Yang, Jian
关键词：Specimen bank; Freshwater mussel; Element; Lake; Monitoring
-
Antibacterial activity and mechanism of luteolin isolated from Lophatherum gracile Brongn. against multidrug-resistant Escherichia coli
作者：Ding, Yahao;Wen, Guilan;Ou, Deyuan;Yang, Jian;Song, Xuqin;Ding, Yahao;Wen, Guilan;Wei, Xingke;Luo, Zhengqin;Yang, Jian;Song, Xuqin;Zhou, Hao;Li, Chunjie
关键词：Lophatherum gracile Brongn.; luteolin; multidrug-resistant Escherichia coli; antibacterial mechanism; untargeted metabolomics
-
The Effect of Artificial Substrate and Carbon Source Addition on Bacterial Diversity and Community Composition in Water in a Pond Polyculture System
作者：Guo, Kun;Shi, Mumin;Ruan, Guoliang;Zhao, Zhigang;Guo, Kun;Shi, Mumin;Huang, Xiaoli;Luo, Liang;Wang, Shihui;Zhang, Rui;Xu, Wei;Zhao, Zhigang;Guo, Kun;Shi, Mumin;Huang, Xiaoli;Luo, Liang;Wang, Shihui;Zhang, Rui;Xu, Wei;Zhao, Zhigang
关键词：artificial substrate; biofloc; 16S rRNA; high-throughput sequencing; microbial community
-
Integrated transcriptome and microRNA analysis reveals molecular responses to high-temperature stress in the liver of American shad (Alosa sapidissima)
作者：Liu, Ying;Liang, Zhengyuan;Li, Yulin;Luo, Mingkun;Dong, Zaijie;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Dong, Zaijie;Feng, Bingbing;Xu, Wei;Wei, Panpan
关键词：Alosa sapidissima; Heat stress; Liver; Genes; miRNAs
-
Copper Stress Causes Shell Morphology Changes in Early Juvenile Anodonta woodiana Based on Geometric-Morphometric Analysis
作者：Yan, Mingjun;Yang, Jian;Chen, Xiubao;Xue, Junren;Liu, Hongbo;Jiang, Tao;Yang, Jian
关键词：Copper; Stress; Chinese pond mussel Anodonta woodiana; Shell shape; Geometric-morphometric; Non-lethal assessment
