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A bZIP transcription factor (CiFD) regulates drought- and low-temperature-induced flowering by alternative splicing in citrus

文献类型： 外文期刊

作者： Ye, Li-Xia 1 ; Wu, Yan-Mei 1 ; Zhang, Jin-Xia 1 ; Zhang, Jin-Xin 1 ; Zhou, Huan 1 ; Zeng, Ren-Fang 1 ; Zheng, Wei-Xuan 1 ; Qiu, Mei-Qi 1 ; Zhou, Jing-Jing 1 ; Xie, Zong-Zhou 1 ; Hu, Chun-Gen 1 ; Zhang, Jin-Zhi 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China

2.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430064, Peoples R China

关键词： alternative splicing; citrus; CiFD; drought; flowering; low temperature

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）

ISSN： 1672-9072

年卷期：

页码：

收录情况： SCI

摘要： Drought and low temperature are two key environmental factors that induce adult citrus flowering. However, the underlying regulation mechanism is poorly understood. The bZIP transcription factor FD is a key component of the florigen activation complex (FAC) which is composed of FLOWERING LOCUS T (FT), FD, and 14-3-3 proteins. In this study, isolation and characterization of CiFD in citrus found that there was alternative splicing (AS) of CiFD, forming two different proteins (CiFD alpha and CiFD beta). Further investigation found that their expression patterns were similar in different tissues of citrus, but the subcellular localization and transcriptional activity were different. Overexpression of the CiFD DNA sequence (CiFD-DNA), CiFD alpha, or CiFD beta in tobacco and citrus showed early flowering, and CiFD-DNA transgenic plants were the earliest, followed by CiFD beta and CiFD alpha. Interestingly, CiFD alpha and CiFD beta were induced by low temperature and drought, respectively. Further analysis showed that CiFD alpha can form a FAC complex with CiFT, Ci14-3-3, and then bind to the citrus APETALA1 (CiAP1) promoter and promote its expression. However, CiFD beta can directly bind to the CiAP1 promoter independently of CiFT and Ci14-3-3. These results showed that CiFD beta can form a more direct and simplified pathway that is independent of the FAC complex to regulate drought-induced flowering through AS. In addition, a bHLH transcription factor (CibHLH96) binds to CiFD promoter and promotes the expression of CiFD under drought condition. Transgenic analysis found that CibHLH96 can promote flowering in transgenic tobacco. These results suggest that CiFD is involved in drought- and low-temperature-induced citrus flowering through different regulatory patterns.

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