Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in black soldier fly Hermetia illucens under different conditions
文献类型： 外文期刊
作者： Ma, Qiuqin 1 ; Gan, Zhending 3 ; Ye, Xiaomei 1 ; Zhang, Ruju 1 ; Han, Ting 1 ; Du, Jing 1 ; Zhu, Fei 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Crop & Livestock Integrat, Nanjing, Peoples R China
3.Nanjing Agr Univ, Coll Anim Sci, Nanjing, Peoples R China
关键词：
genotypes;
期刊名称：INSECT SCIENCE （ 影响因子：3.0； 五年影响因子：3.5 ）
ISSN： 1672-9609
年卷期： 2025 年
页码：
收录情况： SCI
摘要： With the completion of genome sequencing of the black soldier fly (BSF), an increasing number of researchers are focusing on elucidating its robust digestive and absorptive capabilities through genetic and molecular biology methods to enhance its growth performance. However, most genetic and molecular biology studies require gene expression analysis, and the stability of reference genes may change under different experimental conditions. To identify optimal reference genes, we evaluated 11 candidate reference genes (GAPDH, beta-actin, GST, Tubulin alpha-1A, Tubulin alpha-4A, Tubulin beta, RP49, RPL13, EF1-alpha, 18S RNA, and 28S RNA) across various developmental stages, tissues, sexes, and 3 distinct genotypes of Hermetia illucens using RefFinder software, which integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct. The results recommend that beta-Actin, 18S RNA, and RPL13 are suitable for analyzing different developmental stages; RPL13, GST, and RP49 are optimal for different tissues; EF1, RPL13, and GST work best for different sexes; and RPL13, GAPDH, and 18S RNA are ideal for different genotypes. Notably, this study is the first to evaluate the stability of reference genes across 3 BSF genotypes originating from different regions. The findings offer valuable references and fresh insights for molecular biology research on H. illucens.
- 相关文献
作者其他论文 更多>>
-
The role of m6A RNA methylation in a love-hate relationship between porcine rotavirus and host cells
作者：Liang, Yaxu;Zhuo, Ruhao;Peng, Ning;Gan, Zhending;Qi, Jun;Wang, Zhibo;Zhong, Xiang;Zhong, Xiang;Zhu, Xuejiao;Chen, Shuyu;Li, Bin;Zhuo, Ruhao;Huang, Shimeng
关键词：N6-methyladenosine; Porcine rotavirus; Immune; IRF2; IFI44L
-
Protection of humus acids in chicken manure compost by inhibiting aromatic degrading bacteria
作者：Zhang, Ruju;Zhang, Yingpeng;Ye, Xiaomei;Ma, Qiuqin;Xi, Yonglan;Du, Jing;Wang, Cong;Zhu, Fei;Zhang, Ruju;Zhang, Yingpeng;Ye, Xiaomei;Ma, Qiuqin;Xi, Yonglan;Du, Jing;Wang, Cong;Zhu, Fei;Zhang, Ruju;Zhang, Yingpeng;Ye, Xiaomei;Ma, Qiuqin;Xi, Yonglan;Du, Jing;Wang, Cong;Zhu, Fei;Zhou, Jin;Su, Xinya
关键词：Composting; Microbial degradation; Chitosan; Humus
-
Revealing mechanism of phenol-amine reaction to form humus in compost based on high-resolution liquid chromatography mass spectrometry and spectroscopy
作者：Zhou, Jin;Gao, Wenfang;Xie, Lina;Wei, Zimin;Zhou, Jin;Zhang, Ruju;Zhang, Yunxian
关键词：Catechol; Pyrogallol; Glycine; Humification; Humic acid
-
Effect of nanobubble water on medium chain carboxylic acids production in anaerobic digestion of cow manure
作者：Liu, Yang;Ye, Xiaomei;Jiao, Lihua;Zhang, Hongyu;Zhu, Fei;Xi, Yonglan;Liu, Yang;Ye, Xiaomei;Jiao, Lihua;Zhang, Hongyu;Zhu, Fei;Xi, Yonglan;Liu, Yang;Chen, Kequan;Wu, Xiayuan;Xi, Yonglan
关键词：Anaerobic digestion; Chain elongation; Nanobubble water; Electron transport; Fatty acid cycle pathway
-
Composite nanofiber membranes to enhance the performance of high solids anaerobic digestion of organic rural household waste resources
作者：Liu, Yang;Xi, Yonglan;Ye, Xiaomei;Zhang, Yingpeng;Wang, Chengcheng;Jia, Zhaoyan;Cao, Chunhui;Han, Ting;Du, Jing;Kong, Xiangping;Liu, Yang;Xi, Yonglan;Ye, Xiaomei;Zhang, Yingpeng;Wang, Chengcheng;Jia, Zhaoyan;Cao, Chunhui;Han, Ting;Du, Jing;Kong, Xiangping;Liu, Yang;Xi, Yonglan;Ye, Xiaomei;Zhang, Yingpeng;Wang, Chengcheng;Jia, Zhaoyan;Cao, Chunhui;Han, Ting;Du, Jing;Kong, Xiangping;Xi, Yonglan;Chen, Zhongbing
关键词：Methane production; Anaerobic digestion; High solids; Ammonia nitrogen; Nanofiber membrane composites; Prussian blue-like compound nanoparticles
-
Constructing carbon-based materials loaded with MOFs to realize efficient anaerobic digestion of rural organic waste
作者：Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Wang, Chengcheng;Cao, Chunhui;Xi, Yonglan;Xi, Yonglan
关键词：Anaerobic digestion; Metal-organic frameworks; Ammonia nitrogen inhibition; CSTR; Rural organic waste
-
Biogas Slurry Significantly Improved Degraded Farmland Soil Quality and Promoted Capsicum spp. Production
作者：Wang, Zichen;Wang, Jidong;Wang, Zichen;Sanusi, Isaac A.;Kana, Evariste Gueguim;Olaniran, Ademola O.;Wang, Zichen;Ye, Xiaomei;Wang, Zichen;Wang, Jidong;Wang, Zichen;Wang, Jidong
关键词：biogas slurry; plant-environment interaction; soil fertility increase and maintenance; liquid digestate; salt-affected soil; improvement; eco-restoration
