文献类型： 外文期刊

作者： Jia, Zhiying ¹ ; Wu, Nan ⁴ ; Jiang, Xiaona ¹ ; Li, Heng ⁴ ; Sun, Jiaxin ¹ ; Shi, Mijuan ⁴ ; Li, Chitao ¹ ; Ge, Yanlong ¹ ; H ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin, Peoples R China

2.Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin, Peoples R China

3.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Minist Agr, Beijing, Peoples R China

4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

5.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China

6.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China

7.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China

关键词： transcriptome; lncRNA; CyHV-3; resistant immune mechanism; common carp

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.561； 五年影响因子：7.624 ）

ISSN： 1664-3224

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Anti-disease breeding is becoming the most promising solution to cyprinid herpesvirus-3 (CyHV-3) infection, the major threat to common carp aquaculture. Virus challenging studies suggested that a breeding strain of common carp developed resistance to CyHV-3 infection. This study illustrates the immune mechanisms involved in both sensitivity and anti-virus ability for CyHV3 infection in fish. An integrative analysis of the protein-coding genes and long non-coding RNAs (lncRNAs) using transcriptomic data was performed. Tissues from the head kidney of common carp were extracted at days 0 (the healthy control) and 7 after CyHV-3 infection (the survivors) and used to analyze the transcriptome through both Illumina and PacBio sequencing. Following analysis of the GO terms and KEGG pathways involved, the immune-related terms and pathways were merged. To dig out details on the immune aspect, the DEGs were filtered using the current common carp immune gene library. Immune gene categories and their corresponding genes in different comparison groups were revealed. Also, the immunological Gene Ontology terms for lncRNA modulation were retained. The weighted gene co-expression network analysis was used to reveal the regulation of immune genes by lncRNA. The results demonstrated that the breeding carp strain develops a marked resistance to CyHV-3 infection through a specific innate immune mechanism. The featured biological processes were autophagy, phagocytosis, cytotoxicity, and virus blockage by lectins and MUC3. Moreover, the immune-suppressive signals, such as suppression of IL21R on STAT3, PI3K mediated inhibition of inflammation by dopamine upon infection, as well as the inhibition of NLRC3 on STING during a steady state. Possible susceptible factors for CyHV-3, such as ITGB1, TLR18, and CCL4, were also revealed from the non-breeding strain. The results of this study also suggested that Nramp and PAI regulated by LncRNA could facilitate virus infection and proliferation for infected cells respectively, while T cell leukemia homeobox 3 (TLX3), as well as galectin 3 function by lncRNA, may play a role in the resistance mechanism. Therefore, immune factors that are immunogenetically insensitive or susceptible to CyHV-3 infection have been revealed.