您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transient carriage and low-level colonization of orally administrated lytic and temperate phages in the gut of mice

文献类型： 外文期刊

作者： Bao, Hongduo 1 ; Zhang, Hui 1 ; Zhou, Yan 1 ; Zhu, Shujiao 1 ; Pang, Maoda 1 ; Shahin, Khashayar 1 ; Olaniran, Ademola 3 ; Schmidt, Stefan 2 ; Wang, Ran 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safety &, Jiangsu Key Lab Food Qual & Safety,Inst Food Safe, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China

2.Univ KwaZulu Natal, Discipline Microbiol, Sch Life Sci, Coll Agr Engn & Sci, Pietermaritzburg Campus,Private Bag X01, ZA-3201 Pietermaritzburg, South Africa

3.Univ KwaZulu Natal, Discipline Microbiol, Sch Life Sci, Coll Agr Engn & Sci, Westville Campus,Private Bag X54001, ZA-4000 Durban, South Africa

关键词： Lytic phage; Temperate phage; Colonization; Gut; Mice

期刊名称：FOOD PRODUCTION PROCESSING AND NUTRITION （ 影响因子：4.7； 五年影响因子：5.0 ）

ISSN：

年卷期： 2020 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Many studies have shown the efficacy of phage therapy in reducing gastrointestinal pathogens. However, it is unclear whether phages can successfully colonize the gut when administered in an adequate amount for a long time. About 1 x 10(8) PFU/mL of purified lytic phage PA13076 or temperate phage BP96115 were fed daily to mice via drinking water over 31 days, to elucidate the distribution of phages in the gastrointestinal tract. At day 16 and 31, six different segments of the gastrointestinal tract with their contents, including stomach, duodenum, jejunum, ileum, cecum, colon, and fresh feces, were aseptically collected. The phage titers were determined using the double-layered plate method with S. Enteritidis ATCC 13076 or S. Pullorum SPu-109 used as host cells. The results indicated that a small portion of administered phages survived exposure to gastric acid and entered the intestinal tract. The prevalence of phages in the gastrointestinal tract was lower than 1% of the primary phage count. Highest phage titers were detected in the cecum with 10(4) similar to 10(5) PFU/g, and most of the phages were eliminated from the body via feces with 10(6) PFU/g. On day 16 and day 31, the same level of phage titers in different segments of the gastrointestinal tract indicated that the colonization of phages had reached saturation at day 16. These results demonstrate transient phage carriage and low-level colonization of orally administrated lytic and temperate gut phages in mice.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台