Screening of Oral Potential Angiotensin-Converting Enzyme Inhibitory Peptides from Zizyphus jujuba Proteins Based on Gastrointestinal Digestion In Vivo
文献类型： 外文期刊
作者： Gao, Xinchang 1 ; Zhang, Chaoying 3 ; Wang, Ning 1 ; Lin, Jin-Ming 2 ; Dang, Yali 4 ; Zhao, Yufen 1 ;
作者机构： 1.Ningbo Univ, Inst Drug Discovery Technol, Ningbo 315211, Peoples R China
2.Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China
3.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China
4.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Peoples R China
关键词： Zizyphus jujuba; ACE-inhibitory peptides; screening; digestion stability; 3D-QSAR model
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 21 期
页码：
收录情况： SCI
摘要： Plant proteins are a good source of active peptides, which can exert physiological effects on the body. Predicting the possible activity of plant proteins and obtaining active peptides with oral potential are challenging. In this study, the potential activity of peptides from Zizyphus jujuba proteins after in silico simulated gastrointestinal digestion was predicted using the BIOPEP-UWM (TM) database. The ACE-inhibitory activity needs to be further investigated. The actual peptides in mouse intestines after the oral administration of Zizyphus jujuba protein were collected and analyzed, 113 Zizyphus jujuba peptides were identified, and 3D-QSAR models of the ACE-inhibitory activity were created and validated using a training set (34 peptides) and a test set (12 peptides). Three peptides, RLPHV, TVKPGL and KALVAP, were screened using the 3D-QSAR model and were found to bind to the active sites of the ACE enzyme, and their IC50 values were determined. Their values were 6.01, 3.81, and 17.06 mu M, respectively. The in vitro digestion stabilities of the RLPHV, TVKPGL, and KALVAP peptides were 82%, 90%, and 78%. This article provides an integrated method for studying bioactive peptides derived from plant proteins.
- 相关文献
作者其他论文 更多>>
-
One step cleanup of 160 pesticides and veterinary drugs in aquatic products using melamine-based automatic pressure filtration purification method combined with HPLC-MS/MS
作者：Zhou, Ruidong;Kong, Cong;Yang, Guangxin;Huang, Dongmei;Zhou, Ruidong;Wen, Yupeng;Huo, Wendi;Zhang, Chaoying;Sun, Huiwu;Liu, Huan;Li, Jincheng;Zhou, Ruidong;Wen, Yupeng;Huo, Wendi;Zhang, Chaoying;Sun, Huiwu;Liu, Huan;Li, Jincheng;Zhou, Ruidong;Wen, Yupeng;Huo, Wendi
关键词：Aquatic products; Pressure filtration cleanup column; Melamine sponge sorbent; Pesticides and veterinary drugs; Quantitative analysis; Health risk assessment
-
A method for simultaneously and accurately quantify seven quinolones in matrix reference materials by HPLC-MS
作者：Zhang, Chaoying;Wang, Huijiao;Mu, Yingchun;Liu, Huan;Zhang, Chaoying;Wang, Huijiao;Mu, Yingchun;Liu, Huan;Wang, Huijiao
关键词：Antibiotic; Characterization methods; Metrology; Matrix reference material; Value assessment
-
Eco-Friendly Spiking Approach Based on Microfluidics for Preparation of Matrix Reference Materials
作者：Wang, Huijiao;Wang, Huijiao;Liu, Huan;Sun, Huiwu;Zhang, Chaoying;Wang, Huijiao;Liu, Huan;Sun, Huiwu;Zhang, Chaoying
关键词：
-
Improving and controlling the drug loading capacity of seven quinolones in minced fish matrix based on microfluidics
作者：Zhang, Chaoying;Wang, Huijiao;Li, Jincheng;Sun, Huiwu;Liu, Huan;Zhang, Chaoying;Wang, Huijiao;Li, Jincheng;Sun, Huiwu;Liu, Huan;Wang, Huijiao;Li, Jincheng;Liu, Huan
关键词：Microfluidics; Matrix reference material; Drug loading capacity; Minced fish gel microspheres
-
Low fish meal diet supplemented with probiotics ameliorates intestinal barrier and immunological function of Macrobrachium rosenbergii via the targeted modulation of gut microbes and derived secondary metabolites
作者：Zheng, Xiaochuan;Liu, Bo;Wang, Ning;Yang, Jie;Zhou, Qunlan;Sun, Cunxin;Zhao, Yongfeng;Liu, Bo;Wang, Ning;Yang, Jie;Zhou, Qunlan;Zhao, Yongfeng
关键词：Macrobrachium rosenbergii; cottonseed protein concentrate; bacillus coagulans; fish meal; intestinal microbial; secondary metabolites
-
Effect of infection with Aeromonas hydrophila on antioxidant capacity, inflammation response, and apoptosis proteins in Chinese mitten crab (Eriocheir sinensis)
作者：Zheng, Nan;Wang, Ning;Wang, Zhuo-yu;Zhang, Bao-yuan;Wang, Sen;Yao, Qi;Chen, Yu-ke;Wang, Qiu-ju;Zhang, Dong-ming;Zhang, Dong-ming;Abdallah, Ghonimy;Abdallah, Ghonimy;Abdallah, Ghonimy
关键词：Eriocheir sinensis; Aeromonas hydrophila; Antioxidant; Inflammation response; Apoptosis
-
Assessment of fish vulnerability to climate change in the Yellow Sea and Bohai Sea
作者：Chen, Yunlong;Shan, Xiujuan;Jin, Xianshi;Guan, Lisha;Yang, Tao;Dai, Fangqun;Chen, Yunlong;Shan, Xiujuan;Jin, Xianshi;Guan, Lisha;Yang, Tao;Dai, Fangqun;Wang, Ning;Gorfine, Harry
关键词：dominant fish species; fishery management; vulnerability assessments
