文献类型： 外文期刊
作者： Pan, Jin-Min 1 ; Zhu, Ke-Cheng 2 ; Liu, Jun 2 ; Guo, Hua-Yang 2 ; Liu, Bao-Suo 2 ; Zhang, Nan 2 ; Xian, Lin 2 ; Sun, Jin-Hui 4 ; Zhang, Dian-Chang 2 ;
作者机构： 1.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China
3.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China
4.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou, Guangdong, Peoples R China
5.231 Xingang Rd West, Guangzhou 510300, Peoples R China
关键词： Sperm cryopreservation; CASA; Black seabream; Enzyme activity
期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）
ISSN： 0093-691X
年卷期： 2023 年 210 卷
页码：
收录情况： SCI
摘要： In recent years, biotechnology has had a significant impact on the aquaculture industry, particularly in the field of breeding. Molecular selection breeding has emerged as a novel approach to breeding. Reducing the cost of genetic information for individuals with desirable traits after breeding has become an important research direction. Cryopreservation technology allows bypassing time and space constraints in genetic breeding, simplifying broodstock management. This study presents a detailed cryopreservation method for black seabream sperm, evaluating extender type, glucose concentration, cryoprotectant type and concentration, sperm-dilution ratio, and cooling protocols. Sperm motility parameters were analyzed using computer-assisted sperm analysis (CASA) before and after two days of freezing. This involved using an RS solution with a glucose concentration of 15 g/L and adding a 5% final concentration of EG as the sperm cryoprotectant. After mixing the sperm and solution at a ratio of 1:2, we subjected it to 5 min fumigation at 5 cm above the liquid nitrogen surface before plunging it into the nitrogen. Sperm motility reached 85.46 & PLUSMN; 7.32% after two days. Various enzymatic activities showed changes over 20 days post-cryopreservation. This improved cryopreservation protocol for black seabream sperm is beneficial for genetic breeding and reproduction and provides reference for studying the cryodamage mechanisms of black seabream sperm.
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