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Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis

文献类型： 外文期刊

作者： Zhou, Mingjian 1 ; Zhang, Jing 1 ; Shen, Jie 1 ; Zhou, Heng 1 ; Zhao, Didi 1 ; Gotor, Cecilia 2 ; Romero, Luis C. 2 ; Fu, 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Lab Ctr Life Sci, Nanjing 210095, Peoples R China

2.CSIC, Inst Bioquim Vegetal & Fotosintesis, Ave Americo Vespucio 49, Seville 41092, Spain

3.Univ Seville, Ave Americo Vespucio 49, Seville 41092, Spain

4.Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

关键词： hydrogen sulfide; persulfidation; DES1; ABI4; MAPKKK18 transactivation; ABA response

期刊名称：MOLECULAR PLANT （ 影响因子：13.164； 五年影响因子：16.357 ）

ISSN： 1674-2052

年卷期： 2021 年 14 卷 6 期

页码：

收录情况： SCI

摘要： Hydrogen sulfide (H2S) is a signaling molecule that regulates plant hormone and stress responses. The phytohormone abscisic acid (ABA) plays an important role in plant adaptation to unfavorable environmental conditions and induces the persulfidation of L-CYSTEINE DESULFHYDRASE1 (DES1) and the production of H2S in guard cells. However, it remains largely unclear how H2S and protein persulfidation participate in the relay of ABA signals. In this study, we discovered that ABSCISIC ACID INSENSITIVE 4 (ABI4) acts down-stream of DES1 in the control of ABA responses in Arabidopsis. ABI4 undergoes persulfidation at Cys250 that is triggered in a time-dependent manner by ABA, and loss of DES1 function impairs this process. Cys250 and its persulfidation are essential for ABI4 function in the regulation of plant responses to ABA and the H2S donor NaHS during germination, seedling establishment, and stomatal closure, which are abolished in the ABI4(Cys250Ala) mutated variant. Introduction of the ABI4(Cys250Ala) variant into the abi4 des1 mutant did not rescue its hyposensitivity to ABA. Cys250 is critical for the binding of ABI4 to its cognate motif in the promoter of Mitogen-Activated Protein Kinase Kinase Kinase 18 (MAPKKK18), which propagates the MAPK signaling cascade induced by ABA. Furthermore, the DES1-mediated persulfidation of ABI4 enhances the transactivation activity of ABI4 toward MAPKKK18, and ABI4 can bind the DES1 promoter, forming a regulatory loop. Taken together, these findings advance our understanding of a post-translational regulatory mechanism and suggest that ABI4 functions as an integrator of ABA and MAPK signals through a process in which DES1-produced H2S persulfidates ABI4 at Cys250.

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