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Experimental pathogenicity and comparative genome analysis of high- and low-virulence strains of rabbit-origin Pasteurella multocida

文献类型： 外文期刊

作者： Qiu, Rulong 1 ; Wei, Houjun 1 ; Hu, Bo 1 ; Chen, Mengmeng 1 ; Song, Yanhua 1 ; Xu, Weizhong 1 ; Fan, Zhiyu 1 ; Wang, Fang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol,Inst Vet Med, Nanjing 210014, Peoples R China

关键词： Pasteurella multocida; Rabbit; Pathogenicity; Genome; Virulence

期刊名称：COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES （ 影响因子：2.729； 五年影响因子：2.647 ）

ISSN： 0147-9571

年卷期： 2022 年 90-91 卷

页码：

收录情况： SCI

摘要： Pasteurella multocida, the causative pathogen of rabbit pasteurellosis, causes significant economic losses in the commercial rabbit industry. However, the associated pathogenic mechanism of P. multocida remains unclear. The aim of this study is to compare the genomes and pathogenicity of high-and low-virulence strains of P. multocida to advance the current understanding of rabbit pasteurellosis. The high-virulence strain rapidly proliferates in the lung and spleen of infected mice within approximately 9 h, maintaining a high bacterial load until host death. Meanwhile, the low-virulence strain only proliferates in mouse organs for a short time, with the bacterial load beginning to decrease 13 h post-infection. Moreover, the expressions of inflammatory cytokines MCP-1, TNF-alpha, and IL-1 beta are upregulated in all infected mouse lung and spleen tissue, however, the high-virulence strain induced significantly higher expression than the low-virulence strain. Histopathological analysis revealed greater inflammation and tissue lesions in the lung and spleen of mice infected with the high-virulence strain. Two pathogenicity-associated regions unique to the genome of the high-virulence strain harbor approximately 199 genes, including functional genes related to virulence factors, such as lipopolysaccharide biosynthesis, iron acquisition, biosynthesis of outer membrane proteins, and adhesion. These two genomic regions are shared by three previously sequenced, highly virulent P. multocida strains in rabbits. In conclusion, the increased patho-genicity of high-virulence P. multocida may be due to the presence of virulence-associated genes in two unique genomic regions, resulting in strong proliferative activity, significant inflammation, and pathological lesions in the mouse model. These findings provide important insights regarding the pathogenic mechanism underlying rabbit pasteurellosis.

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