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An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease

文献类型： 外文期刊

作者： Sheng, Cong 1 ; Li, Xuan 1 ; Xia, Shengge 1 ; Zhang, Yimai 1 ; Yu, Ze 1 ; Tang, Cheng 1 ; Xu, Le 1 ; Wang, Zhaoyun 2 ; Zhang, Xin 4 ; Zhou, Tong 3 ; Nie, Pingping 5 ; Baig, Ayesha 6 ; Niu, Dongdong 1 ; Zhao, Hongwei 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Lab Biointeract & Crop Hlth, Nanjing 210095, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety, Nanjing 210095, Peoples R China

4.Shanxi Agr Univ, Inst Ind Crops, Taiyuan 030000, Peoples R China

5.Zaozhuang Univ, Coll Life Sci, Zaozhuang 277000, Peoples R China

6.COMSATS Univ Islamabad, Dept Biotechnol, Abbottabad Campus, Abbottabad, Pakistan

关键词： glucose; miR1875; OsAGO2; OsHXK1; OsPRMT5; rice blast resistance

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 4 期

页码：

收录情况： SCI

摘要： Rice ARGONAUTE2 (OsAGO2) is a core component of the rice RNA-induced silencing complex (RISC), which is repressed by Magnaporthe oryzae (M. oryzae) infection. Whether and how OsAGO2-mediated gene silencing plays a role in rice blast resistance and which sRNAs participate in this process are unknown. Our results indicate that OsAGO2 is a key immune player that manipulates rice defense responses against blast disease. OsAGO2 associates with the 24-nt miR1875 and binds to the promoter region of HEXOKINASE1 (OsHXK1), which causes DNA methylation and leads to gene silencing. Our multiple genetic evidence showed that, without M. oryzae infection, OsAGO2/miR1875 RISC promoted OsHXK1 promoter DNA methylation and OsHXK1 silencing; after M. oryzae infection, the reduced OsAGO2/miR1875 led to a relatively activated OsHXK1 expression. OsHXK1 acts as a positive regulator of blast disease resistance that OsHXK1-OE rice exhibited enhanced resistance, whereas Cas9-Oshxk1 rice showed reduced resistance against M. oryzae infection. OsHXK1 may function through its sugar sensor activity as glucose induced defense-related gene expression and reactive oxygen species (ROS) accumulation in Nipponbare and OsHXK1-OE but not in Cas9-Oshxk1 rice. OsAGO2 itself is delicately regulated by OsPRMT5, which senses M. oryzae infection and attenuates OsAGO2-mediated gene silencing through OsAGO2 arginine methylation. Our study reveals an OsPRMT5-OsAGO2/miR1875-OsHXK1 regulatory module that fine tunes the rice defense response to blast disease.

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