Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116
文献类型： 外文期刊
作者： Zhu, Jinyu 1 ; Tan, Zhongfang 2 ; Zhang, Zhong 3 ; Shi, Xianming 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Zhengzhou Univ, Coll Agr Sci, Zhengzhou 450001, Peoples R China
3.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
关键词： Exopolysaccharide; Lactobacillus curvatus; Antioxidation activity; Antibacterial activity; Anti-biofilm formation activity
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 210 卷
页码：
收录情况： SCI
摘要： This study aimed to investigate the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116 from the fish Gymnocypris przewalskii. The purified EPS, denoted as EPS-1, was mainly composed of glucose and mannose at a relative molar ratio of 1:1.05 with molecular weight of 31.9 kDa. The chemical structure of EPS-1 was consisted of -> 2)-alpha-D-Manp-(1 ->, -> 4)-alpha-D-Manp-(1 ->, -> 3,6)-alpha-D-Manp-(1 ->, T beta-D-Glcp-(1 ->, -> 6)-beta-D-Glcp-(1 ->, and -> 3)-beta-D-Glcp-(1 -> glycosidic bonds. A sheet-like structure of dried EPS-1 was determined by scanning electron microscope (SEM), whilst a peak-shaped structure of EPS-1 was observed by atomic force microscope (AFM). The degradation temperature of EPS-1 was determined as 300.21 degrees C using thermogravimetric analysis (TGA). Moreover, the antioxidant capacity of EPS-1 at a concentration of 5.0 mg/mL against DPPH and ABTS was 84.50% and 92.53%, respectively. Furthermore, EPS-1 exhibited acceptable bacteriostatic efficacy against S. Enteritidis, E. coli, and S.aureus with significant inhibition of S. Enteritidis biofilm formation.
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