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Early supplementation with Lactobacillus plantarum in liquid diet modulates intestinal innate immunity through toll-like receptor 4-mediated mitogen-activated protein kinase signaling pathways in young piglets challenged with Escherichia coli K88

文献类型： 外文期刊

作者： Yang, Kuanmin M. 1 ; Zhu, Cui 3 ; Wang, Li 1 ; Cao, Shuting T. 1 ; Yang, Xuefen F. 1 ; Gao, Kaiguo G. 1 ; Jiang, Zongy 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Key Lab Anim Nutr & Feed South China, State Key Lab Livestock & Poultry Breeding, Guangzhou, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Maoming Branch, Guangdong Lab Lingnan Modern Agr, Inst Anim Sci,Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

3.Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Peoples R China

关键词： enterotoxigenic Escherichia coil K88; intestinal innate immunity; Lactobacillus piantarum; mitogen-activated protein kinase; piglets; toll-like receptor 4

期刊名称：JOURNAL OF ANIMAL SCIENCE （ 影响因子：3.159； 五年影响因子：2.912 ）

ISSN： 0021-8812

年卷期： 2021 年 99 卷 6 期

页码：

收录情况： SCI

摘要： This study was conducted to investigate the effects of early supplementation during 4 to 18 d of age with Lactobacillus plantarum (LP) in liquid diets on intestinal innate immune response in young piglets infected with enterotoxigenic Escherichia coli (ETEC) K88. Seventy-two barrow piglets at 4 d old were assigned to basal or LP-supplemented liquid diet (5 x 10(10) CFU.kg(-1)). On day 15, piglets from each group were orally challenged with either ETEC K88 (1 x 10(8) CFU.kg(-1)) or the same amount of phosphate-buffered saline. The intestinal mucosa, mesenteric lymph node (MLN), and spleen samples were collected on day 18. Here, we found that LP pretreatment significantly decreased the mRNA relative expression of inflammatory cytokines (interleukin [IL]-1 beta, IL-8, and tumor necrosis factor-alpha), porcine beta-defensin 2 (pBD-2), and mucins (MUCI and MUC4) in the jejunal mucosa in piglets challenged with ETEC K88 (P < 0.05). Moreover, LP significantly decreased the ileal mucosa mRNA relative expression of IL-8 and MUC4 in young piglets challenged with ETEC K88 (P < 0.05). Furthermore, the piglets of the LP + ETEC K88 group had lower protein levels of IL-8, secretory immunoglobulin A, pBD-2, and MUC4 in the jejunal mucosa than those challenged with ETEC K88 (P < 0.05). Besides, LP supplementation reduced the percentage of gamma/delta T cells receptor (gamma delta TCR) and CD172a(+) (SWC3(+)) cells in MLN and the percentage of gamma delta TCR cells in the spleen of young piglets after the ETEC K88 challenge. Supplementation with LP in liquid diets prevented the upregulated protein abundance of toll-like receptor (TLR) 4, phosphorylation-p38, and phosphorylation-extracellular signal-regulated protein kinases in the jejunal mucosa induced by ETEC K88 (P < 0.05). In conclusion, LP supplementation in liquid diet possesses anti-inflammatory activity and modulates the intestinal innate immunity during the early life of young piglets challenged with ETEC K88, which might be attributed to the suppression of TLR4-mediated mitogen-activated protein kinase signaling pathways. Early supplementation with LP in liquid diets regulates the innate immune response, representing a promising immunoregulation strategy for maintaining intestinal health in weaned piglets.

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