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Integrated transcriptomics and metabolomics analysis provide insight into the resistance response of rice against brown planthopper

文献类型： 外文期刊

作者： Shi, Shaojie 1 ; Zha, Wenjun 1 ; Yu, Xinying 1 ; Wu, Yan 1 ; Li, Sanhe 1 ; Xu, Huashan 1 ; Li, Peide 1 ; Li, Changyan 1 ; Liu, Kai 1 ; Chen, Junxiao 1 ; Yang, Guocai 1 ; Chen, Zhijun 1 ; Wu, Bian 1 ; Wan, Bingliang 1 ; Zhou, Lei 1 ; You, Aiqing 1 ;

作者机构： 1.Hubei Acad Agr Sci, Food Crops Inst, Lab Crop Mol Breeding, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan, Peoples R China

2.Hubei Hongshan Lab, Wuhan, Peoples R China

关键词： rice; brown planthopper; plant-insect interaction; multi-omics analysis; Bph30

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： IntroductionThe brown planthopper (Nilaparvata lugens Stal, BPH) is one of the most economically significant pests of rice. The Bph30 gene has been successfully cloned and conferred rice with broad-spectrum resistance to BPH. However, the molecular mechanisms by which Bph30 enhances resistance to BPH remain poorly understood. MethodsHere, we conducted a transcriptomic and metabolomic analysis of Bph30-transgenic (BPH30T) and BPH-susceptible Nipponbare plants to elucidate the response of Bph30 to BPH infestation. ResultsTranscriptomic analyses revealed that the pathway of plant hormone signal transduction enriched exclusively in Nipponbare, and the greatest number of differentially expressed genes (DEGs) were involved in indole 3-acetic acid (IAA) signal transduction. Analysis of differentially accumulated metabolites (DAMs) revealed that DAMs involved in the amino acids and derivatives category were down-regulated in BPH30T plants following BPH feeding, and the great majority of DAMs in flavonoids category displayed the trend of increasing in BPH30T plants; the opposite pattern was observed in Nipponbare plants. Combined transcriptomics and metabolomics analysis revealed that the pathways of amino acids biosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis and flavonoid biosynthesis were enriched. The content of IAA significantly decreased in BPH30T plants following BPH feeding, and the content of IAA remained unchanged in Nipponbare. The exogenous application of IAA weakened the BPH resistance conferred by Bph30. DiscussionOur results indicated that Bph30 might coordinate the movement of primary and secondary metabolites and hormones in plants via the shikimate pathway to enhance the resistance of rice to BPH. Our results have important reference significance for the resistance mechanisms analysis and the efficient utilization of major BPH-resistance genes.

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