Natural variations in a barley aldehyde oxidase 1 gene affect seed germination and malting quality
文献类型： 外文期刊
作者： Xu, Le 1 ; Wang, Peng 1 ; Zhang, Xiaoqi 2 ; Zhang, Qisen 3 ; Wang, Penghao 2 ; Xu, Yanhao 4 ; Li, Chengdao 2 ; Zhang, Wenying 1 ;
作者机构： 1.Yangtze Univ, Coll Agr, Engn Res Ctr Ecol & Agr Use Wetland, MARA Key Lab Sustainable Crop Prod Middle Reaches, Jingzhou 434023, Hubei, Peoples R China
2.Murdoch Univ, Western Barley Genet Alliance, Perth, WA 6150, Australia
3.Australian Export Grains Innovat Ctr, Perth, WA 6151, Australia
4.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan 430064, Hubei, Peoples R China
关键词： Dormancy; Germination; Malting quality; Aldehyde oxidase; Barley (Hordeum vulgare L.)
期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）
ISSN： 2095-5421
年卷期： 2025 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Multiple phytohormones, including gibberellin (GA), abscisic acid (ABA), and indole-3-acetic acid (IAA), regulate seed germination. In this study, a barley aldehyde oxidase 1 (HvAO1) gene was identified, which is located near the SD2 (seed dormancy 2) region at the telomeric end of chromosome 5H. A doubled- haploid population (AC Metcalfe/Baudin) was used to characterize HvAO1 and validated its association with seed germination and malting quality. Aldehyde oxidase is predicted to catalyse the oxidation of various aldehydes, such as indoleacetaldehyde and abscisic aldehyde, into IAA and ABA, which is the final step of IAA/ABA biogenesis. This process influences the final IAA/ABA concentration in the seed, affecting the seed dormancy. Sequence analysis revealed substantial variations in the HvAO1 promoter regions between AC Metcalfe and Baudin. The combining seed germination tests, genetic variation analysis, gene expression, and phytohormone measurements showed that Baudin, which displays strong seed dormancy, has a specific sequence variation in the promoter region of the HvAO1 gene. This variation is associated with a higher expression level of the HvAO1 gene and an increased level of ABA than those in AC Metcalfe, which shows weak dormancy and lacks this sequence variation. In addition to its strong effect on the SD2 gene, HvAO1 shows excellent potential to fine-tune malting quality and seed dormancy, as evidenced by genotyping with HvAO1-specific markers, dormancy phenotypes, and malting quality. Our findings provide a new strategy for introducing favourable HvAO1 alleles to achieve the desired level of seed dormancy and high malting quality in barley. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- 相关文献
作者其他论文 更多>>
-
Evolutionary analysis of anthocyanin biosynthetic genes: insights into abiotic stress adaptation
作者：Buitrago, Sebastian;Pan, Rui;Zhang, Wenying;Yang, Xinsun;Wang, Lianjun
关键词：Abiotic stress; Chalcone isomerase; Gene family evolution; Reactive oxygen species (ROS); Phenylpropanoids
-
Bioinformatics and Expression Analysis of CHI Gene Family in Sweet Potato
作者：Wu, Yaqin;Zhang, Wenying;Pan, Rui;Wu, Yaqin;Jin, Xiaojie;Wang, Lianjun;Lei, Jian;Chai, Shasha;Yang, Xinsun;Wang, Chong;Yang, Xinsun
关键词：sweet potato; chalcone isomerase; abiotic stress; bioinformatics analysis; expression analysis
-
Transcriptional convergence after repeated duplication of an amino acid transporter gene leads to the independent emergence of the black husk/pericarp trait in barley and rice
作者：Li, Bo;Zhang, Shuo;Guo, Ying;Jiao, Chunhai;Xu, Yanhao;Li, Bo;Zhang, Shuo;Guo, Ying;Jiao, Chunhai;Xu, Yanhao;Jia, Yong;Li, Chengdao;Xu, Le;Long, Zhoukai;Wang, Rong;Zhang, Wenying;Li, Chengdao
关键词：barley; black husk/pericarp; Blp; melanin; convergent evolution; rice
-
Integrated Transcriptional and Metabolomic Analysis of Factors Influencing Root Tuber Enlargement during Early Sweet Potato Development
作者：Wu, Yaqin;Jin, Xiaojie;Wang, Lianjun;Lei, Jian;Chai, Shasha;Yang, Xinsun;Wu, Yaqin;Zhang, Wenying;Wang, Chong
关键词：sweet potato; root development; transcriptomics; metabolomics; weighted gene co-expression network analysis (WGCNA)
-
Physiological, Epigenetic, and Transcriptome Analyses Provide Insights into the Responses of Wheat Seedling Leaves to Different Water Depths under Flooding Conditions
作者：Li, Bo;Zhang, Shuo;Zhu, Zhanwang;Guo, Ying;Jiao, Chunhai;Xu, Yanhao;Li, Bo;Zhang, Shuo;Zhu, Zhanwang;Guo, Ying;Jiao, Chunhai;Xu, Yanhao;Hua, Wei;Xu, Le;Yang, Caixian;Zhou, Meixue
关键词：Triticum aestivum L.; DNA methylation; transcriptional analysis; water depth; flooding stress
-
The wheat TaSAMS10 improves survival rate under waterlogging stress by inducing ethylene synthesis and early flowering
作者：Cheng, Bingyun;Feng, Xiaobing;Buitrago, Sebastian;Wan, Kui;Xu, Le;Pan, Rui;Zhang, Wenying;Xu, Yanhao
关键词：Triticum aestivum; Hypoxia stress; S-adenosylmethionine synthetase; Ethylene synthesis; Early flowering
-
Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene
作者：Qin, Dandan D.;Liu, Rui;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Cheng, Hongna;Dong, Jing;Qin, Dandan D.;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Dong, Jing;Qin, Dandan D.;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Dong, Jing;Liu, Rui;Dong, Guoqing;Xu, Le;Cheng, Hongna;Guo, Ganggang;Li, Chengdao
关键词：barley; BSR-seq; TCP; multiple stem nodes and spikes and dwarf; fine-mapping
