Antioxidant Activity of Gracilaria lemaneiformis Polysaccharide Degradation Based on Nrf-2/Keap-1 Signaling Pathway in HepG2 Cells with Oxidative Stress Induced by H2O2
文献类型： 外文期刊
作者： Long, Xiaoshan 1 ; Hu, Xiao 2 ; Pan, Chuang 2 ; Xiang, Huan 2 ; Chen, Shengjun 2 ; Qi, Bo 2 ; Liu, Shucheng 1 ; Yang, Xianqing 2 ;
作者机构： 1.Guangdong Ocean Univ, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Guangdon, Zhanjiang 524088, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural, Guangzhou 510300, Peoples R China
3.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
4.Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China
关键词： G; lemaneiformis; polysaccharide; degradation; free radicals; antioxidant; signaling pathway
期刊名称：MARINE DRUGS （ 影响因子：6.085； 五年影响因子：6.044 ）
ISSN：
年卷期： 2022 年 20 卷 9 期
页码：
收录情况： SCI
摘要： The objective of this research was to investigate the antioxidant activity of Gracilaria lemaneiformis polysaccharide degradation and its underlying mechanism involved in the Nrf-2/Keap-1 signaling pathway in HepG2 cells with oxidative stress induced by H2O2. The result of the scavenging ability of free radicals showed that GLP-HV (polysaccharide degraded by H2O2-vitamin C (Vc)) performed a better scavenging ability than GLP (G. lemaneiformis polysaccharide). Moreover, the scavenging ability of polysaccharide to these free radicals from strong to weak was as follows: superoxide radical, ferric ion, ABTS(+), and DPPH radical, and their IC50 values were 3.56 +/- 0.0028, 4.97 +/- 0.18, 9.62 +/- 0.35, and 23.85 +/- 1.78 mg/mL, respectively. Furthermore, GLP-HV obviously relieved oxidative stress in HepG2 cells, which strengthened the activity of T-AOC, CAT, GSH-PX, and SOD, and diminished the intensity of MDA, intracellular ROS, and calcium ion based on the Nrf-2/Keap-1 signaling pathway. The PCR result revealed that polysaccharide upregulated the expression of the genes Nrf-2, HO-1, NQO-1, and ZO-1 and downregulated Keap-1. The correlation between chemical properties and antioxidant mechanism of GLP-HV was evaluated via a heat map. The results illustrated that reducing sugar and active groups presented a positive correlation, and molecular weight and viscosity exhibited a negative relation with antioxidant activity.
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