文献类型： 外文期刊
作者： Wang, Zhijun 1 ; Cai, Danfeng 1 ; Ju, Xing 1 ; Li, Kan 1 ; Liang, Sisi 4 ; Fang, Meixia 4 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Peoples R China
2.Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Local Joint Engn Res Ctr Livestock Breeding, Guangzhou 510642, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Natl Local Joint Engn Res Ctr Livestock Breeding, Guangzhou 510642, Peoples R China
4.Jinan Univ, Dept Lab Anim Sci, Med Coll, Guangzhou 510632, Peoples R China
关键词： betaine; myogenesis; N6-Methyladenosine; mRNA-Seq; chicken
期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）
ISSN： 2076-2615
年卷期： 2022 年 12 卷 19 期
页码：
收录情况： SCI
摘要： Simple Summary Betaine is a healthy source of methyl and glycine and a widely used feed additive to promote animal growth. Previous studies mainly focused on its anti-osmotic pressure, anti-inflammatory, and growth-promoting effect in vitro. Still, poultry's growth-promoting mechanism and regulation of muscle cells remain unknown. In this study, we found that a low concentration of betaine could inhibit myoblast cell proliferation but promote myotube formation, suggesting that the growth-promoting effect of betaine was achieved through the differentiation and fusion of myotubes. In addition, RNA sequencing found a series of betaine-affected genes, which could provide a theoretical basis for explaining betaine's regulation on chicken myogenesis in vitro. Betaine is trimethylglycine and a universal methyl donor which could provide methyl and glycine for cells and animals. As a new star in epigenetics, N6-Methyladenosine has been reported to regulate multiple biological activities, but the regulatory mechanism of betaine on N6-Methyladenosine as well as myogenesis was little studied. In this study, we treated chicken primary myoblast cells with different concentrations of betaine (0, 10, 25, and 50 mmol/L) and found that myoblast cell proliferation was inhibited, although the cell cycle was promoted in the S phase by betaine, where the myotube area was increased as well as the differentiation marker genes MyoD, MyoG, MyHC, Myomarker, and Ckm. RNA sequencing obtained a total of 61 differentially expressed genes (DEGs); DEGs caused by 50 mmol/L betaine were mainly enriched in the regulation of skeletal muscle tissue regeneration and some amino acid metabolic processes. The gene expression pattern trends of all DEGs were mainly clustered into 2 profiles, with the increase in betaine concentration, the gene expression pattern either increased or decreased continuously. Overall, a low concentration betaine can increase the N6-Methyladenosine modification level and myotube area but depresses myoblast cell proliferation in vitro.
- 相关文献
作者其他论文 更多>>
-
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
作者：Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua
关键词：TMEM182; Fat formation; Metabolomics; Lipidomics
-
Using multiomics to explore the weight differences between genders in Muscovy ducks
作者：Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Zhu, Weijian;Ji, Congliang
关键词：Muscovy duck; sexual dimorphism; growth and development; RNA-seq; 16S rRNA sequencing technology
-
Live chicken body fat measurement technology based on bio-electrical impedance
作者：Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Deng, Yuelin
关键词：BIA; Electrical impedance; Fat content; Broilers
-
LncEDCH1 g.1703613 T>C regulates chicken carcass traits by targeting miR-196-2-3p
作者：Yuan, Rongshuai;Cai, Bolin;Ma, Manting;Zhao, Changbin;Xian, Yuanrong;Nie, Qinghua;Zhang, Xiquan;Zhang, Dexiang;Zhang, Dexiang
关键词：LncEDCH1; miR-196-2-3p; single nucleotide polymorphism; carcass trait; chicken
-
Weighted single-step GWAS identified candidate genes associated with carcass traits in a Chinese yellow-feathered chicken population
作者：Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Pan, Rongyang;Qi, Lin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen
关键词：Chinese yellow-feathered chicken; weighted single-step GWAS; carcass traits; SNP
-
MicroRNA expression profile of chicken jejunum in different time points Eimeria maxima infection
作者：Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Hanotte, Olivier;Tuli, Merga Daba;Hanotte, Olivier
关键词：microRNA; expression profile; chicken jejunum; host immunity; Eimeria maxima
-
Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism
作者：Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Zhang, Zihao;Lin, Shudai;Meng, Xun;Meng, Xun
关键词：Muscle antibody array; Myoblast differentiation; Energy metabolism regulation
