文献类型： 外文期刊
作者： Zhang, Hui 1 ; Gong, Huanran 1 ; Zhou, Xueping 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects MOA, Hangzhou 310029, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China
期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity (>98%) and have more than 97% sequence identity with TYLCV-IL[IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solarium lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annu-um. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum. We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-beta in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.
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