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Precise location of two novel linear epitopes on the receptor-binding domain surface of MERS-CoV spike protein recognized by two different monoclonal antibodies

文献类型： 外文期刊

作者： Wang, Pan 1 ; Ding, Peiyang 3 ; Wei, Qiang 2 ; Liu, Hongliang 3 ; Liu, Yunchao 2 ; Li, Qingmei 2 ; Xing, Yunrui 2 ; Li, Ge 2 ; Zhou, Enmin 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

5.Yangzhou Univ, Jiangsu Co Innovat Ctr Prevent & Control Importan, Yangzhou, Peoples R China

关键词： MERS-CoV RBD; Monoclonal antibody; Novel linear B cell epitopes

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 195 卷

页码：

收录情况： SCI

摘要： Middle East respiratory syndrome coronavirus (MERS-CoV) is a coronavirus which can cause severe human respiratory diseases with a fatality rate of almost 36%.& nbsp;In this study, we report the generation, characterization and epitope mapping of several monoclonal antibodies against the spike receptor-binding domain (RBD) of MERS-CoV. Two monoclonal antibodies ((4C7) and (6E8)) that can react with linearized RBD have been selected for subsequent identification of RBD mAb-binding epitopes. Two distinct novel linear epitopes, 423FTCSQIS(429) and (546)SPLEGGGWL(554),were precisely located at the outermost surface of RBD by dot-blot hybridization and ELISAs. Multiple sequence alignment analysis showed that these two peptides were highly conserved. Alanine (A)-scanning mutagenesis demonstrated that residues 423F, 428I, and 429S are the crucial residues for the linear epitope (423)FTCSQIS(429) while residues 548L, 550G, 553W, 554L for epitope (546)SPLEGGGWL(554). These findings may be helpful for further understanding of the function of RBD protein and the development of subsequent diagnosis and detection methods.

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