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Development and application of multiplex PCR for the rapid identi fi cation of four Fusarium spp. associated with Fusarium crown rot in wheat

文献类型： 外文期刊

作者： Deng, Siyi 1 ; Chang, Wei 1 ; Liu, Quanke 4 ; Zhao, Youfu 5 ; Liu, Jun 1 ; Wang, Hua 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan, Peoples R China

2.Hubei Key Lab Crop Dis Insect Pests & Weeds Contro, Wuhan, Peoples R China

3.Minist Agr, Key Lab Integrated Pest Management Crops Cent Chin, Wuhan, Peoples R China

4.Gen Plant Protect Stn Hubei Prov, Wuhan, Peoples R China

5.Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA USA

关键词： Fusarium crown rot; Fusarium spp.; Multiplex PCR; Identi fi cation; Speci fi city; Whole genome sequence comparison

期刊名称：PEERJ （ 影响因子：2.3； 五年影响因子：2.8 ）

ISSN： 2167-8359

年卷期： 2024 年 12 卷

页码：

收录情况： SCI

摘要： Fusarium crown rot (FCR), caused by Fusarium spp., is a devastating disease in wheat growing areas. Previous studies have shown that FCR is caused by co -infection of F. graminearum, F. pseudograminearum, F. proliferatum and F. verticillioides in Hubei Province, China. In this study, a method was developed to simultaneously detected DNAs of F. graminearum, F. pseudograminearum, F. proliferatum and F. verticillioides that can ef fi ciently differentiate them. Whole genome sequence comparison of these four Fusarium spp. was performed and a 20 bp sequence was designed as an universal upstream primer. Speci fi c downstream primers of each pathogen was also designed, which resulted in a 206, 482, 680, and 963 bp amplicon for each pathogen, respectively. Multiplex PCR speci fi cally identi fi ed F. graminearum, F. pseudograminearum, F. proliferatum and F. verticillioides but not from other 46 pathogens, and the detection limit of target pathogens is about 100 pg/ m l. Moreover, we accurately determined the FCR pathogen species in wheat samples using the optimized multiplex PCR method. These results demonstrate that the multiplex PCR method established in this study can ef fi ciently and rapidly identify F. graminearum , F. pseudograminearum , F. proliferatum , and F. verticillioides , which should provide technical support for timely and targeted prevention and control of FCR.

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