Development of a Multiplex PCR Assay for the Detection of Tomato Wilt Caused by Coinfection of Fusarium brachygibbosum, Fusarium oxysporum, and Ralstonia solanacearum Based on Comparative Genomics
文献类型: 外文期刊
作者: Liu, Jun 1 ; Deng, Siyi 1 ; Chang, Wei 1 ; Yu, Dazhao 1 ; Wang, Hua 1 ;
作者机构: 1.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan 430064, Peoples R China
2.Hubei Key Lab Crop Dis Insect Pests & Weeds Contro, Wuhan 430064, Peoples R China
3.Minist Agr, Key Lab Integrated Pest Management Crops Cent Chin, Wuhan 430064, Peoples R China
关键词: comparative genomics; detection; Fusarium brachygibbosum; Fusarium oxysporum; multiplex PCR; Ralstonia solanacearum; tomato wilt
期刊名称:PLANT DISEASE ( 2022影响因子:4.5; 五年影响因子:5.0 )
ISSN: 0191-2917
年卷期: 2024 年 108 卷 5 期
收录情况: SCI
摘要: Tomato is consumed worldwide as fresh or processed food products. However, soilborne diseases of tomato plants caused by coinfection of various pathogens result in great economic losses to the tomato industry. It is difficult to accurately identify and diagnose soilborne diseases of tomato plants caused by pathogen complexes. In this study, we investigated field diseases of tomato plants by pathogen isolation and molecular identification and found that tomato wilt was caused by coinfection of Fusarium brachygibbosum, F. oxysporum, and Ralstonia solanacearum. Therefore, developing a method for simultaneous detection of DNA from F. brachygibbosum, F. oxysporum, and R. solanacearum is of great importance to efficiently and accurately monitor disease development at different growth stages of tomato plants. In this study, we performed a comparative genomic analysis of F. brachygibbosum, F. oxysporum, and R. solanacearum and determined the primer sets for simultaneous detection of DNA from these target pathogens. Then, we tested the reagent and condition parameters of multiplex PCR, including primers, dNTP and Mg2+ concentrations, and annealing temperatures, to determine the optimal parameters of a multiplex PCR system. We evaluated the specificity, sensitivity, and stability of the multiplex PCR system based on the optimized reaction conditions. The multiplex PCR system can specifically identify 13 target pathogens from 57 different fungal and bacterial pathogens, at the lower detection limit of the three target pathogens at concentrations of 100 pg/mu l. In addition, we can accurately identify the three pathogens in tomato plants using the optimized multiplex PCR method. These results demonstrated that the multiplex PCR method developed in this study can simultaneously detect DNA from F. brachygibbosum, F. oxysporum, and R. solanacearum in a single PCR system to accurately identify and diagnose the pathogen causing tomato wilt.
- 相关文献
作者其他论文 更多>>
-
Development and application of multiplex PCR for the rapid identi fi cation of four Fusarium spp. associated with Fusarium crown rot in wheat
作者:Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Liu, Quanke;Zhao, Youfu
关键词:Fusarium crown rot; Fusarium spp.; Multiplex PCR; Identi fi cation; Speci fi city; Whole genome sequence comparison
-
A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew
作者:Li, Miaomiao;Zhang, Huaizhi;Xiao, Huixin;Zhu, Keyu;Wu, Qiuhong;Xie, Jingzhong;Chen, Yongxing;Qiu, Dan;Guo, Guanghao;Lu, Ping;Li, Beibei;Dong, Lei;Li, Wenling;Cui, Xuejia;Li, Lingchuan;Tian, Xiubin;Li, Yiwen;Dong, Lingli;Zhao, Yusheng;Liu, Zhiyong;Zhu, Keyu;Chen, Yongxing;Li, Beibei;Dong, Lei;Li, Wenling;Cui, Xuejia;Li, Lingchuan;Zhao, Yusheng;Liu, Zhiyong;Shi, Wenqi;Yang, Lijun;Yu, Dazhao;Zhang, Dong;Wang, Yong;Yuan, Chengguo;Nevo, Eviatar;Fahima, Tzion;Li, Hongjie;Liu, Zhiyong
关键词:
-
Combined transcriptome and metabolome analyses reveal the effects of selenium on the growth and quality of Lilium lancifolium
作者:Jiang, Xiaogang;Zhou, Wuxian;Li, Darong;Wang, Hua;Yang, Yuying;You, Jinwen;Liu, Haihua;Ai, Lunqiang;Zhang, Meide
关键词:Lilium lancifolium; selenium; soluble sugar; amino acid; soluble protein
-
Transcriptome and metabolome analyses reveal novel insights into the seed germination of Michelia chapensis, an endangered species in China
作者:Zhou, Wuxian;Duan, Yuanyuan;Jiang, Xiaogang;Tan, Xuhui;Li, Qin;Wang, Hua;Zhang, Meide;Zhou, Wuxian;Duan, Yuanyuan;Jiang, Xiaogang;Tan, Xuhui;Li, Qin;Wang, Hua;Zhang, Meide;Zhang, Yajuan
关键词:Michelia chapensis; Seed germination; Plant hormone signal transduction; Starch and sucrose metabolism; Energy supply; Biosynthesis of secondary metabolites
-
First specific detection and validation of tomato wilt caused by Fusarium brachygibbosum using a PCR assay
作者:Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Liu, Quanke
关键词:Tomato wilt; Fusarium brachygibbosum; Identification; Specificity; Sensitivity; Whole genome sequence comparison
-
TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.
作者:Jiang, Xiaogang;Zhou, Wuxian;Wang, Hua;You, Jinwen;Zhang, Meide;Liu, Wenlu
关键词:Codonopsis tangshen Oliv; proteome; continuous cropping; biological process
-
Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen
作者:Jiang, Xiaogang;Zhou, Wuxian;Wang, Hua;You, Jinwen;Zhang, Meide;Wassie, Misganaw;Ma, Guangjing
关键词:Codonopsis tangshen; heat-stress; heat-responsive genes; RNA-sequencing; transcriptome