文献类型： 外文期刊

作者： Li, Miaomiao ¹ ; Zhang, Huaizhi ¹ ; Xiao, Huixin ¹ ; Zhu, Keyu ¹ ; Shi, Wenqi ³ ; Zhang, Dong ⁴ ; Wang, Yong ⁵ ; Yang, Lijun ³ ; Wu, Qiuhong ¹ ; Xie, Jingzhong ¹ ; Chen, Yongxing ¹ ; Qiu, Dan ¹ ; Guo, Guanghao ¹ ; Lu, Ping ¹ ; Li, Beibei ¹ ; Dong, Lei ¹ ; Li, Wenling ¹ ; Cui, Xuejia ¹ ; Li, Lingchuan ¹ ; Tian, Xiubin ¹ ; Yuan, Chengguo ⁶ ; Li, Yiwen ¹ ; Yu, Dazhao ³ ; Nevo, Eviatar ⁷ ; Fahima, Tzion ⁷ ; Li, Hongjie ⁸ ; Dong, Lingli ¹ ; Zhao, Yusheng ¹ ; Liu, Zhiyong ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Innovat Acad Seed Design, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China

2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China

3.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan, Peoples R China

4.Beijing PlantTech Biotechnol Co Ltd, Beijing, Peoples R China

5.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China

6.Hebei Gaoyi Seeds Farm, Gaoyi, Hebei, Peoples R China

7.Univ Haifa, Inst Evolut, Har Hakarmel, Haifa, Israel

8.Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Lab Crop Mol Breeding, Beijing, Peoples R China

9.Hainan Seed Ind Lab, Sanya, Hainan, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.7； 五年影响因子：16.1 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance. Powdery mildew is a fungal leaf disease that reduces yield and grain quality in susceptible wheat varieties. Here, the authors report the cloning of the wild emmer wheat originated powdery mildew resistance gene Pm36 as a membrane associated tandem kinase and its possible resistance mechanism.