Preparation of chitooligosaccharides with a low degree of polymerization and anti-microbial properties using the novel chitosanase AqCsn1
文献类型： 外文期刊
作者： Xia, Chengyao 1 ; Li, Ding 2 ; Qi, Mengyi 1 ; Wang, Yanxin 1 ; Zhang, Yue 1 ; Yang, Yiheng 1 ; Hu, Zejia 1 ; Du, Xin 1 ; Zhao, Yuqiang 5 ; Yu, Kuai 1 ; Huang, Yan 1 ; Li, Zhoukun 1 ; Ye, Xianfeng 1 ; Cui, Zhongli 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Agr Environm Microbiol, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
5.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Peoples R China
关键词： Chitosanase; Chitosan; Chitooligosaccharides; Agricultural diseases
期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.6； 五年影响因子：1.6 ）
ISSN： 1046-5928
年卷期： 2023 年 203 卷
页码：
收录情况： SCI
摘要： Chitosanases hydrolyze chitosan into chitooligosaccharides (COSs) with various biological activities, which are widely employed in many areas including plant disease management. In this study, the novel chitosanase AqCsn1 belonging to the glycoside hydrolase family 46 (GH46) was cloned from Aquabacterium sp. A7-Y and heterolo-gously expressed in Escherichia coli BL21 (DE3). AqCsn1 displayed the highest hydrolytic activity towards chi-tosan with 95% degree of deacetylation at 40 degrees C and pH 5.0, with a specific activity of 13.18 U/mg. Product analysis showed that AqCsn1 hydrolyzed chitosan into (GlcN)2 and (GlcN)3 as the main products, demonstrating an endo-type cleavage pattern. Evaluation of antagonistic activity showed that the hydrolysis products of AqCsn1 suppress the mycelial growth of Magnaporthe oryzae and Phytophthora sojae in a concentration-dependent manner, and the inhibition rate of P. sojae reached 39.82% at a concentration of 8 g/L. Our study demon-strates that AqCsn1 and hydrolysis products with a low degree of polymerization might have potential appli-cations in the biological control of agricultural diseases.
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