Isolation and characterization of 112 SNP markers in Yellowfin seabream (Acanthopagrus latus) using RAD sequencing
文献类型： 外文期刊
作者： Zhu, Ke-Cheng 1 ; Li, Ya-Ling 2 ; Wu, Wei-Bin 1 ; Liu, Bao-Suo 1 ; Guo, Hua-Yang 1 ; Zhang, Nan 1 ; Guo, Liang 1 ; Zhang, Dian-Chang 1 ;
作者机构： 1.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
3.Guangdong Prov Engn Technol Res Ctr Marine Biol S, Guangzhou 510300, Peoples R China
4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ctr, Sanya 572018, Peoples R China
5.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China
6.231 Xingang Rd West, Guangzhou 510300, Peoples R China
关键词： Acanthopagrus latus; SNP; RAD; Breeding; Enhancement and releasing
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.991； 五年影响因子：0.897 ）
ISSN： 1877-7252
年卷期：
页码：
收录情况： SCI
摘要： The yellowfin seabream (Acanthopagrus latus Houttuyn 1782) is a commercially and ecologically important Sparidae species in the northern South China Sea. However, environmental pollution and overfishing result in serious decline of seabream natural population and germplasm degradation in recent years. In the present study, to promote the molecular-assisted selection (MAS), population selection, and enhancement and releasing of A. latus, the 112 novel single nucleotide polymorphism (SNP) markers were isolated by restriction-site associated DNA (RAD) sequencing, and then the polymorphism of these SNP markers were verified in 36 wild individuals. The results showed that the observed heterozygosity (Ho) and expected heterozygosity (H-E) varied from 0.0278 to 0.6389 and 0.0540 to 0.4996, respectively. The PIC value and minor allele frequency (MAF) were between 0.0526 and 0.3748 and 0.0278-0.4861, respectively. Moreover, the F-IS value ranged from - 0.2941 to 0.9449. Thirty-seven loci revealed noteworthy deviations from the Hardy-Weinberg equilibrium (HWE) after Bonferroni correction (p < 0.05), but no prominent linkage disequilibrium was detected. These novel developed SNPs will be useful for breeding, genetic diversity analysis, enhancement and releasing studies of seabream.
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