您好，欢迎访问新疆农垦科学院 机构知识库！
新疆农垦科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

1 alpha,25(OH)(2)D-3 Promotes the Autophagy of Porcine Ovarian Granulosa Cells as a Protective Mechanism against ROS through the BNIP3/PINK1 Pathway

文献类型： 外文期刊

作者： Wang, Shiyou 1 ; Yao, Qichun 1 ; Zhao, Fan 1 ; Cui, Wenfei 1 ; Price, Christopher A. 2 ; Wang, Yifan 1 ; Lv, Jing 1 ; Tang, Hong 3 ; Jiang, Zhongliang 1 ;

作者机构： 1.Northwestern A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet, Breeding & Reprod Shaanxi Prov, Xianyang 712100, Peoples R China

2.Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertil, St Hyacinthe, PQ J2S 7E4, Canada

3.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Med, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China

关键词： 1 alpha; 25(OH)(2)D-3; porcine granulosa cells; autophagy; ROS; signaling pathway

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 5 期

页码：

收录情况： SCI

摘要： Vitamin D (VD) is one of the important nutrients required by livestock; however, VD deficiency is reported to be widespread. Earlier studies have suggested a potential role for VD in reproduction. Studies on the correlation between VD and sow reproduction are limited. The aim of the current study was aimed to determine the role of 1,25-dihydroxy vitamin D-3 (1 alpha,25(OH)(2)D-3) on porcine ovarian granulosa cells (PGCs) in vitro to provide a theoretical basis for improving the reproductive efficiency of sows. We used chloroquine (autophagy inhibitor) and reactive oxygen species (ROS) scavenger N-acetylcysteine in conjunction with 1 alpha,25(OH)(2)D-3 to explore the effect on PGCs. The results showed that 10 nM of 1 alpha,25(OH)(2)D-3 increased PGC viability and ROS content. In addition, 1 alpha,25(OH)(2)D-3 induces PGC autophagy according to the gene transcription and protein expression levels of LC3, ATG7, BECN1, and SQSTM1 and promotes the generation of autophagosomes. 1 alpha,25(OH)(2)D-3-induced autophagy affects the synthesis of E-2 and P-4 in PGCs. We investigated the relationship between ROS and autophagy, and the results showed that 1 alpha,25(OH)(2)D-3-induced ROS promoted PGC autophagy. The ROS-BNIP3-PINK1 pathway was involved in PGC autophagy induced by 1 alpha,25(OH)(2)D-3. In conclusion, this study suggests that 1 alpha,25(OH)(2)D-3 promotes PGC autophagy as a protective mechanism against ROS via the BNIP3/PINK1 pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农垦科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台