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Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus x female Channa maculata ) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)

文献类型： 外文期刊

作者： Deng, Huiling 1 ; Zheng, Shucheng 1 ; Li, Yingying 1 ; Mo, Xubing 1 ; Zhao, Jian 1 ; Yin, Jiyuan 1 ; Shi, Cunbin 1 ; Wang, Qing 1 ; Wang, Yingying 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou 510380, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.City Univ Hong Kong, Jockey Club Sch Anim Med & Life Sci, Dept Infect Dis & Publ Hlth, Key Lab Marine Pollut,Kowloon, Hong Kong, Peoples R China

4.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Peoples R China

关键词： CAMK cell line; Hybrid snakehead; Virology; Hybrid snakehead rhabdovirus

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 1096-4959

年卷期： 2024 年 273 卷

页码：

收录情况： SCI

摘要： Hybrid snakehead (male Channa argus x female Channa maculata) is an emerging fish breed with increasing production levels. However, infection with hybrid snakehead rhabdovirus (HSHRV) critically affects hybrid snakehead farming. In this study, a fish cell line called CAMK, derived from the kidneys of hybrid snakehead, was established and characterized. CAMK cells exhibited the maximum growth rate at 28 degrees C in Leibovitz's-15 medium supplemented with 10% fetal bovine serum(FBS). Karyotyping revealed diploid chromosomes in 54% of the cells at the 50th passage (2n = 66), and 16S rRNA sequencing validated that CAMK cells originated fromhybrid snakehead, and the detection of kidney-specific antibodies suggested that it originated from kidney. .The culture was free from mycoplasma contamination, and the green fluorescent protein gene was effectively transfected into CAMK cells, indicating their potential use for in vitro gene expression investigations. Furthermore, qRT-PCR and immunofluorescence analysis revealed that HSHRV could replicate in CAMK cells, indicating that the cells were susceptible to the virus. Transmission electron microscopy revealed that the viral particles had bullet-like morphology. The replication efficiency of HSHRV was 107.33 TCID50/mL. Altogether, we successfully established and characterized a kidney cell line susceptible to the virus. These findings provide a valuable reference for further genetic and virological studies.

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